論文 - 詳細
| RRC ID | 35461 |
|---|---|
| 著者 | Wenk M, Ba Q, Erichsen V, MacInnes K, Wiese H, Warscheid B, Koch HG. |
| タイトル | A universally conserved ATPase regulates the oxidative stress response in Escherichia coli. |
| ジャーナル | J Biol Chem |
| Abstract |
YchF is an evolutionarily conserved ATPase of unknown function. In humans, the YchF homologue hOla1 appears to influence cell proliferation and was found to be up-regulated in many tumors. A possible involvement in regulating the oxidative stress response was also suggested, but details on the underlying mechanism are lacking. For gaining insight into YchF function, we used Escherichia coli as a model organism and found that YchF overexpression resulted in H(2)O(2) hypersensitivity. This was not caused by transcriptional or translational down-regulation of H(2)O(2)-scavenging enzymes. Instead, we observed YchF-dependent inhibition of catalase activity and a direct interaction with the major E. coli catalase KatG. KatG inhibition was dependent on the ATPase activity of YchF and was regulated by post-translational modifications, most likely including an H(2)O(2)-dependent dephosphorylation. We furthermore showed that YchF expression is repressed by the transcription factor OxyR and further post-translationally modified in response to H(2)O(2). In summary, our data show that YchF functions as a novel negative regulator of the oxidative stress response in E. coli. Considering the available data on hOla1, YchF/Ola1 most likely execute similar functions in bacteria and humans, and their up-regulation inhibits the ability of the cells to scavenge damaging reactive oxygen species. |
| 巻・号 | 287(52) |
| ページ | 43585-98 |
| 公開日 | 2012-12-21 |
| DOI | 10.1074/jbc.M112.413070 |
| PII | S0021-9258(20)41754-5 |
| PMID | 23139412 |
| PMC | PMC3527945 |
| MeSH | Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism* Catalase / genetics Catalase / metabolism Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* GTP-Binding Proteins / genetics GTP-Binding Proteins / metabolism Humans Hydrogen Peroxide / pharmacology* Oxidants / pharmacology* Oxidative Stress / drug effects* Oxidative Stress / genetics Phosphorylation / drug effects Phosphorylation / genetics Protein Processing, Post-Translational / drug effects Protein Processing, Post-Translational / genetics Repressor Proteins / genetics Repressor Proteins / metabolism |
| IF | 4.238 |
| 引用数 | 17 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | JW1194-KC JW3933-KC |