Reference - Detail
| RRC ID | 35462 |
|---|---|
| Author | Butala M, Sonjak S, Kamenšek S, Hodošček M, Browning DF, Žgur-Bertok D, Busby SJ. |
| Title | Double locking of an Escherichia coli promoter by two repressors prevents premature colicin expression and cell lysis. |
| Journal | Mol Microbiol |
| Abstract |
The synthesis of Eschericha coli colicins is lethal to the producing cell and is repressed during normal growth by the LexA transcription factor, which is the master repressor of the SOS system for repair of DNA damage. Following DNA damage, LexA is inactivated and SOS repair genes are induced immediately, but colicin production is delayed and induced only in terminally damaged cells. The cause of this delay is unknown. Here we identify the global transcription repressor, IscR, as being directly responsible for the delay in colicin K expression during the SOS response, and identify the DNA target for IscR at the colicin K operon promoter. Our results suggest that, IscR stabilizes LexA at the cka promoter after DNA damage thus, preventing its cleavage and inactivation, and this cooperation ensures that suicidal colicin K production is switched on only as a last resort. A similar mechanism operates at the regulatory region of other colicins and, hence, we suggest that many promoters that control the expression of 'lethal' genes are double locked. |
| Volume | 86(1) |
| Pages | 129-39 |
| Published | 2012-10-1 |
| DOI | 10.1111/j.1365-2958.2012.08179.x |
| PMID | 22812562 |
| MeSH | Bacterial Proteins / metabolism* Bacteriolysis Base Sequence Colicins / biosynthesis* Escherichia coli / genetics* Escherichia coli / metabolism* Escherichia coli / physiology Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial* Models, Biological Molecular Sequence Data Promoter Regions, Genetic* Protein Binding Serine Endopeptidases / metabolism* Transcription Factors / metabolism* |
| IF | 3.418 |
| Times Cited | 24 |
| WOS Category | MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | DH5α? ME9062(BW25113) JW2515-KC JW0824-KC JW2947-KC |