論文 - 詳細
| RRC ID | 35468 |
|---|---|
| 著者 | Kumagai A, Ando R, Miyatake H, Greimel P, Kobayashi T, Hirabayashi Y, Shimogori T, Miyawaki A. |
| タイトル | A bilirubin-inducible fluorescent protein from eel muscle. |
| ジャーナル | Cell |
| Abstract |
The fluorescent protein toolbox has revolutionized experimental biology. Despite this advance, no fluorescent proteins have been identified from vertebrates, nor has chromogenic ligand-inducible activation or clinical utility been demonstrated. Here, we report the cloning and characterization of UnaG, a fluorescent protein from Japanese eel. UnaG belongs to the fatty-acid-binding protein (FABP) family, and expression in eel is restricted to small-diameter muscle fibers. On heterologous expression in cell lines or mouse brain, UnaG produces oxygen-independent green fluorescence. Remarkably, UnaG fluorescence is triggered by an endogenous ligand, bilirubin, a membrane-permeable heme metabolite and clinical health biomarker. The holoUnaG structure at 1.2 Å revealed a biplanar coordination of bilirubin by reversible π-conjugation, and we used this high-affinity and high-specificity interaction to establish a fluorescence-based human bilirubin assay with promising clinical utility. UnaG will be the prototype for a versatile class of ligand-activated fluorescent proteins, with applications in research, medicine, and bioengineering. |
| 巻・号 | 153(7) |
| ページ | 1602-11 |
| 公開日 | 2013-6-20 |
| DOI | 10.1016/j.cell.2013.05.038 |
| PII | S0092-8674(13)00644-2 |
| PMID | 23768684 |
| MeSH | Amino Acid Sequence Animals Bilirubin / metabolism Cloning, Molecular Eels / metabolism* Fish Proteins / chemistry Fish Proteins / genetics* Fish Proteins / isolation & purification* Fish Proteins / metabolism Green Fluorescent Proteins / chemistry Green Fluorescent Proteins / genetics Green Fluorescent Proteins / isolation & purification* Green Fluorescent Proteins / metabolism Molecular Sequence Data Muscles / metabolism Sequence Alignment |
| IF | 38.637 |
| 引用数 | 126 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 263 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 遺伝子材料 | UnaG/pcDNA3-FLAG (RDB15703) UnaG/pGEX-2T (RDB15704) pET-His UNAG (RDB13504) |