RRC ID 35511
著者 Ogasawara H, Shinohara S, Yamamoto K, Ishihama A.
タイトル Novel regulation targets of the metal-response BasS-BasR two-component system of Escherichia coli.
ジャーナル Microbiology (Reading)
Abstract The BasS-BasR two-component system is known as an iron- and zinc-sensing transcription regulator in Escherichia coli, but so far only a few genes have been identified to be under the direct control of phosphorylated BasR. Using Genomic SELEX (systematic evolution of ligands by exponential enrichment) screening, we have identified a total of at least 38 binding sites of phosphorylated BasR on the E. coli genome, and based on the BasR-binding sites, have predicted more than 20 novel targets of regulation. By DNase I footprint analysis for high-affinity BasR-binding sites, a direct repeat of a TTAAnnTT sequence was identified as the BasR box. Transcription regulation in vivo of the target genes was confirmed after Northern blot analysis of target gene mRNAs from both wild-type E. coli and an otherwise isogenic basR deletion mutant. The BasR regulon can be classified into three groups of genes: group 1 includes the genes for the formation and modification of membrane structure; group 2 includes genes for modulation of membrane functions; and group 3 includes genes for stress-response cell functions, including csgD, the master regulator of biofilm formation.
巻・号 158(Pt 6)
ページ 1482-1492
公開日 2012-6-1
DOI 10.1099/mic.0.057745-0
PMID 22442305
MeSH Base Sequence Binding Sites Escherichia coli / chemistry Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / chemistry Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial* Metals / metabolism Molecular Sequence Data Protein Binding Transcription, Genetic
IF 2.138
引用数 35
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) ME9062(BW25113) JW4074-KC