論文 - 詳細
| RRC ID | 35511 |
|---|---|
| 著者 | Ogasawara H, Shinohara S, Yamamoto K, Ishihama A. |
| タイトル | Novel regulation targets of the metal-response BasS-BasR two-component system of Escherichia coli. |
| ジャーナル | Microbiology (Reading) |
| Abstract |
The BasS-BasR two-component system is known as an iron- and zinc-sensing transcription regulator in Escherichia coli, but so far only a few genes have been identified to be under the direct control of phosphorylated BasR. Using Genomic SELEX (systematic evolution of ligands by exponential enrichment) screening, we have identified a total of at least 38 binding sites of phosphorylated BasR on the E. coli genome, and based on the BasR-binding sites, have predicted more than 20 novel targets of regulation. By DNase I footprint analysis for high-affinity BasR-binding sites, a direct repeat of a TTAAnnTT sequence was identified as the BasR box. Transcription regulation in vivo of the target genes was confirmed after Northern blot analysis of target gene mRNAs from both wild-type E. coli and an otherwise isogenic basR deletion mutant. The BasR regulon can be classified into three groups of genes: group 1 includes the genes for the formation and modification of membrane structure; group 2 includes genes for modulation of membrane functions; and group 3 includes genes for stress-response cell functions, including csgD, the master regulator of biofilm formation. |
| 巻・号 | 158(Pt 6) |
| ページ | 1482-1492 |
| 公開日 | 2012-6-1 |
| DOI | 10.1099/mic.0.057745-0 |
| PMID | 22442305 |
| MeSH | Base Sequence Binding Sites Escherichia coli / chemistry Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / chemistry Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial* Metals / metabolism Molecular Sequence Data Protein Binding Transcription, Genetic |
| IF | 2.138 |
| 引用数 | 35 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME9062(BW25113) JW4074-KC |