Reference - Detail
| RRC ID | 35516 |
|---|---|
| Author | Yamamoto S, Suzuki K. |
| Title | Development of a reinforced Ti-eviction plasmid useful for construction of Ti plasmid-free Agrobacterium strains. |
| Journal | J Microbiol Methods |
| Abstract |
For curing stable Ti plasmids from Agrobacterium strains, we developed a new Ti-eviction plasmid. The plasmid contains two antitoxin genes, which originated from two toxin-antitoxin systems in Ti plasmids. The plasmid facilitated production of Ti-less cells in seven tested strains, and was removed easily by simple counter-selection from the cells. |
| Volume | 89(1) |
| Pages | 53-6 |
| Published | 2012-4-1 |
| DOI | 10.1016/j.mimet.2012.01.019 |
| PII | S0167-7012(12)00037-1 |
| PMID | 22326751 |
| MeSH | Agrobacterium / genetics* Bacterial Toxins Genomic Instability Microbial Viability Plant Tumor-Inducing Plasmids* Selection, Genetic Sequence Deletion |
| IF | 1.707 |
| Times Cited | 2 |
| WOS Category | MICROBIOLOGY BIOCHEMICAL RESEARCH METHODS |
| Resource | |
| Prokaryotes E. coli | NA |