Reference - Detail
| RRC ID | 35519 |
|---|---|
| Author | Hiraishi T, Yamashita K, Sakono M, Nakanishi J, Tan LT, Sudesh K, Abe H, Maeda M. |
| Title | Display of functionally active PHB depolymerase on Escherichia coli cell surface. |
| Journal | Macromol Biosci |
| Abstract |
The display of PHB depolymerase (PhaZ(RpiT1) ) from R. pickettii T1 on the surface of E. coli JM109 cells is realized using OprI of P. aeruginosa as the anchoring motif. The fusion protein is stably expressed and its surface localization is verified by immunofluorescence microscopy. The displayed PhaZ(RpiT1) retains its cleaving ability for soluble substrates as well as its ability to adsorb to the PHB surface, and also remains catalycically active in the degradation of insoluble polyester materials, in spite of the possible suppression of the enzyme movement on the polymer surface. The results demonstrate that PhaZ(RpiT1) -displaying E. coli shows potential for use as a whole-cell biocatalyst for the production of (R)-3-hydroxybutyrate monomers from insoluble PHB materials. |
| Volume | 12(2) |
| Pages | 218-24 |
| Published | 2012-2-1 |
| DOI | 10.1002/mabi.201100273 |
| PMID | 22095689 |
| MeSH | 3-Hydroxybutyric Acid / biosynthesis* Bacterial Proteins / genetics Bacterial Proteins / metabolism* Biocatalysis Carboxylic Ester Hydrolases / genetics Carboxylic Ester Hydrolases / metabolism* Cloning, Molecular Electrophoresis, Polyacrylamide Gel Escherichia coli / enzymology* Escherichia coli / genetics Gene Expression Hydroxybutyrates / metabolism* Kinetics Lipoproteins / genetics Lipoproteins / metabolism* Microscopy, Fluorescence Plasmids Polyesters / metabolism* Pseudomonas aeruginosa / chemistry Pseudomonas aeruginosa / genetics Ralstonia pickettii / chemistry Ralstonia pickettii / genetics Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Substrate Specificity Surface Properties |
| IF | 3.416 |
| Times Cited | 5 |
| WOS Category | POLYMER SCIENCE MATERIALS SCIENCE, BIOMATERIALS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Prokaryotes E. coli | pVUB3 |