論文 - 詳細
| RRC ID | 35527 |
|---|---|
| 著者 | Chen SS, Sperling E, Silverman JM, Davis JH, Williamson JR. |
| タイトル | Measuring the dynamics of E. coli ribosome biogenesis using pulse-labeling and quantitative mass spectrometry. |
| ジャーナル | Mol Biosyst |
| Abstract |
The ribosome is an essential organelle responsible for cellular protein synthesis. Until recently, the study of ribosome assembly has been largely limited to in vitro assays, with few attempts to reconcile these results with the more complex ribosome biogenesis process inside the living cell. Here, we characterize the ribosome synthesis and assembly pathway for each of the E. coli ribosomal protein (r-protein) in vivo using a stable isotope pulse-labeling timecourse. Isotope incorporation into assembled ribosomes was measured by quantitative mass spectrometry (qMS) and fit using steady-state flux models. Most r-proteins exhibit precursor pools ranging in size from 0% to 7% of completed ribosomes, and the sizes of these individual r-protein pools correlate well with the order of r-protein binding in vitro. Additionally, we observe anomalously large precursor pools for specific r-proteins with known extra-ribosomal functions, as well as three r-proteins that apparently turnover during steady-state growth. Taken together, this highly precise, time-dependent proteomic qMS approach should prove useful in future studies of ribosome biogenesis and could be easily extended to explore other complex biological processes in a cellular context. |
| 巻・号 | 8(12) |
| ページ | 3325-34 |
| 公開日 | 2012-10-30 |
| DOI | 10.1039/c2mb25310k |
| PMID | 23090316 |
| PMC | PMC3501348 |
| MeSH | Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / analysis Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Mass Spectrometry Nitrogen Isotopes Proteomics RNA, Ribosomal / chemistry RNA, Ribosomal / genetics RNA, Ribosomal / metabolism Ribosomal Proteins / chemistry Ribosomal Proteins / metabolism* Ribosome Subunits, Large, Bacterial / metabolism* Ribosome Subunits, Small, Bacterial / metabolism* Ribosomes / metabolism* |
| IF | 3.336 |
| 引用数 | 24 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | |