| RRC ID |
35541
|
| Author |
Liu H, Ramos KR, Valdehuesa KN, Nisola GM, Lee WK, Chung WJ.
|
| Title |
Biosynthesis of ethylene glycol in Escherichia coli.
|
| Journal |
Appl Microbiol Biotechnol
|
| Abstract |
Ethylene glycol (EG) is an important platform chemical with steadily expanding global demand. Its commercial production is currently limited to fossil resources; no biosynthesis route has been delineated. Herein, a biosynthesis route for EG production from D-xylose is reported. This route consists of four steps: D-xylose → D-xylonate → 2-dehydro-3-deoxy-D-pentonate → glycoaldehyde → EG. Respective enzymes, D-xylose dehydrogenase, D-xylonate dehydratase, 2-dehydro-3-deoxy-D-pentonate aldolase, and glycoaldehyde reductase, were assembled. The route was implemented in a metabolically engineered Escherichia coli, in which the D-xylose → D-xylulose reaction was prevented by disrupting the D-xylose isomerase gene. The most efficient construct produced 11.7 g L(-1) of EG from 40.0 g L(-1) of D-xylose. Glycolate is a carbon-competing by-product during EG production in E. coli; blockage of glycoaldehyde → glycolate reaction was also performed by disrupting the gene encoding aldehyde dehydrogenase, but from this approach, EG productivity was not improved but rather led to D-xylonate accumulation. To channel more carbon flux towards EG than the glycolate pathway, further systematic metabolic engineering and fermentation optimization studies are still required to improve EG productivity.
|
| Volume |
97(8)
|
| Pages |
3409-17
|
| Published |
2013-4-1
|
| DOI |
10.1007/s00253-012-4618-7
|
| PMID |
23233208
|
| MeSH |
Biosynthetic Pathways / genetics*
Escherichia coli / genetics*
Escherichia coli / metabolism*
Ethylene Glycol / metabolism*
Metabolic Engineering / methods
Xylose / metabolism
|
| IF |
3.53
|
| Times Cited |
52
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| Resource |
| Prokaryotes E. coli |
JW1412-KC |
