| RRC ID |
35542
|
| Author |
Liu H, Ramos KR, Valdehuesa KN, Nisola GM, Malihan LB, Lee WK, Park SJ, Chung WJ.
|
| Title |
Metabolic engineering of Escherichia coli for biosynthesis of D-galactonate.
|
| Journal |
Bioprocess Biosyst Eng
|
| Abstract |
D-galactose is an attractive substrate for bioconversion. Herein, Escherichia coli was metabolically engineered to convert D-galactose into D-galactonate, a valuable compound in the polymer and cosmetic industries. D-galactonate productions by engineered E. coli strains were observed in shake flask cultivations containing 2 g L(-1) D-galactose. Engineered E. coli expressing gld coding for galactose dehydrogenase from Pseudomonas syringae was able to produce 0.17 g L(-1) D-galactonate. Inherent metabolic pathways for assimilating both D-galactose and D-galactonate were blocked to enhance the production of D-galactonate. This approach finally led to a 7.3-fold increase with D-galactonate concentration of 1.24 g L(-1) and yield of 62.0 %. Batch fermentation in 20 g L(-1) D-galactose of E. coli ∆galK∆dgoK mutant expressing the gld resulted in 17.6 g L(-1) of D-galactonate accumulation and highest yield of 88.1 %. Metabolic engineering strategy developed in this study could be useful for industrial production of D-galactonate.
|
| Volume |
37(3)
|
| Pages |
383-91
|
| Published |
2014-3-1
|
| DOI |
10.1007/s00449-013-1003-6
|
| PMID |
23820824
|
| MeSH |
Base Sequence
Cloning, Molecular
Culture Media
DNA Primers
Escherichia coli / genetics
Escherichia coli / metabolism*
Galactose Dehydrogenases / genetics
Hydrogen-Ion Concentration
Magnetic Resonance Spectroscopy
Molecular Structure
Pseudomonas syringae / enzymology
Sugar Acids / chemistry
Sugar Acids / metabolism*
|
| IF |
2.419
|
| Times Cited |
5
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
ENGINEERING, CHEMICAL
|
| Resource |
| Prokaryotes E. coli |
ME9062(BW25113)
JW0740-KC(galK) |
