Reference - Detail
| RRC ID | 35542 |
|---|---|
| Author | Liu H, Ramos KR, Valdehuesa KN, Nisola GM, Malihan LB, Lee WK, Park SJ, Chung WJ. |
| Title | Metabolic engineering of Escherichia coli for biosynthesis of D-galactonate. |
| Journal | Bioprocess Biosyst Eng |
| Abstract |
D-galactose is an attractive substrate for bioconversion. Herein, Escherichia coli was metabolically engineered to convert D-galactose into D-galactonate, a valuable compound in the polymer and cosmetic industries. D-galactonate productions by engineered E. coli strains were observed in shake flask cultivations containing 2 g L(-1) D-galactose. Engineered E. coli expressing gld coding for galactose dehydrogenase from Pseudomonas syringae was able to produce 0.17 g L(-1) D-galactonate. Inherent metabolic pathways for assimilating both D-galactose and D-galactonate were blocked to enhance the production of D-galactonate. This approach finally led to a 7.3-fold increase with D-galactonate concentration of 1.24 g L(-1) and yield of 62.0 %. Batch fermentation in 20 g L(-1) D-galactose of E. coli ∆galK∆dgoK mutant expressing the gld resulted in 17.6 g L(-1) of D-galactonate accumulation and highest yield of 88.1 %. Metabolic engineering strategy developed in this study could be useful for industrial production of D-galactonate. |
| Volume | 37(3) |
| Pages | 383-91 |
| Published | 2014-3-1 |
| DOI | 10.1007/s00449-013-1003-6 |
| PMID | 23820824 |
| MeSH | Base Sequence Cloning, Molecular Culture Media DNA Primers Escherichia coli / genetics Escherichia coli / metabolism* Galactose Dehydrogenases / genetics Hydrogen-Ion Concentration Magnetic Resonance Spectroscopy Molecular Structure Pseudomonas syringae / enzymology Sugar Acids / chemistry Sugar Acids / metabolism* |
| IF | 2.419 |
| Times Cited | 5 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY ENGINEERING, CHEMICAL |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | ME9062(BW25113) JW0740-KC(galK) |