RRC ID 35555
著者 Ferreira E, Giménez R, Aguilera L, Guzmán K, Aguilar J, Badia J, Baldomà L.
タイトル Protein interaction studies point to new functions for Escherichia coli glyceraldehyde-3-phosphate dehydrogenase.
ジャーナル Res Microbiol
Abstract Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is considered a multifunctional protein with defined functions in numerous mammalian cellular processes. GAPDH functional diversity depends on various factors such as covalent modifications, subcellular localization, oligomeric state and intracellular concentration of substrates or ligands, as well as protein-protein interactions. In bacteria, alternative GAPDH functions have been associated with its extracellular location in pathogens or probiotics. In this study, new intracellular functions of Escherichia coli GAPDH were investigated following a proteomic approach aimed at identifying interacting partners using in vivo formaldehyde cross-linking followed by mass spectrometry. The identified proteins were involved in metabolic processes, protein synthesis and folding or DNA repair. Some interacting proteins were also identified in immunopurification experiments in the absence of cross-linking. Pull-down experiments and overlay immunoblotting were performed to further characterize the interaction with phosphoglycolate phosphatase (Gph). This enzyme is involved in the metabolism of 2-phosphoglycolate formed in the DNA repair of 3'-phosphoglycolate ends generated by bleomycin damage. We show that interaction between Gph and GAPDH increases in cells challenged with bleomycin, suggesting involvement of GAPDH in cellular processes linked to DNA repair mechanisms.
巻・号 164(2)
ページ 145-54
公開日 2013-1-1
DOI 10.1016/j.resmic.2012.11.002
PII S0923-2508(12)00177-5
PMID 23195894
MeSH Centrifugation Cross-Linking Reagents / metabolism Escherichia coli / enzymology* Escherichia coli Proteins / metabolism* Formaldehyde / metabolism Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism* Immunoblotting Mass Spectrometry Protein Binding Protein Interaction Mapping*
IF 3.217
引用数 13
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) CV-49(MC4100)? CV-70(Top10)? CV-4(BL21(DE3))? ME5305(AG1)?