論文 - 詳細
| RRC ID | 35562 |
|---|---|
| 著者 | Armshaw P, Pembroke JT. |
| タイトル | Generation and analysis of an ICE R391 deletion library identifies genes involved in the element encoded UV-inducible cell-sensitising function. |
| ジャーナル | FEMS Microbiol Lett |
| Abstract |
ICE R391, a prototype member of the SXT/R391 family of site-specific integrative conjugative elements (ICEs), frequently isolated from enterobacterial pathogens, exhibits an unusual, recA-dependent, UV-inducible, cell-sensitising function. This significantly decreases postirradiation cell survival rates in Escherichia coli host cells, a trait that would at first appear to be counterproductive in terms of adaptation to stress conditions. Construction and screening of a complete ICE R391 deletion library in E. coli identified three ICE R391 genes, orfs90/91, encoding a putative transcriptional enhancer, and orf43, encoding a putative type IV secretion system outer membrane-associated conjugative transfer protein, in the cell-sensitising function. Cloning and complementation of these genes confirmed their involvement in UV sensitising. Expression of both orfs90/91 and orf43 in wild-type E. coli indicated that orf43 encodes a cytotoxic gene product upon up-regulation. Deletion of the orf43 homologue in SXT, s050, also abolished its associated UV sensitisation. We hypothesise that ICE R391 and other members of the SXT/R391 family display decreased survival rates upon exposure to UV irradiation through the induction of orf43. |
| 巻・号 | 342(1) |
| ページ | 45-53 |
| 公開日 | 2013-5-1 |
| DOI | 10.1111/1574-6968.12107 |
| PMID | 23413978 |
| MeSH | Escherichia coli / genetics* Escherichia coli / radiation effects* Gene Deletion* Gene Library* Genetic Complementation Test Interspersed Repetitive Sequences* Microbial Viability / radiation effects* Ultraviolet Rays* |
| IF | 1.987 |
| 引用数 | 9 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(大腸菌) | pBAD33 |