Reference - Detail
| RRC ID | 35580 |
|---|---|
| Author | Kochanowski K, Volkmer B, Gerosa L, Haverkorn van Rijsewijk BR, Schmidt A, Heinemann M. |
| Title | Functioning of a metabolic flux sensor in Escherichia coli. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
Regulation of metabolic operation in response to extracellular cues is crucial for cells' survival. Next to the canonical nutrient sensors, which measure the concentration of nutrients, recently intracellular "metabolic flux" was proposed as a novel impetus for metabolic regulation. According to this concept, cells would have molecular systems ("flux sensors") in place that regulate metabolism as a function of the actually occurring metabolic fluxes. Although this resembles an appealing concept, we have not had any experimental evidence for the existence of flux sensors and also we have not known how these flux sensors would work in detail. Here, we show experimental evidence that supports the hypothesis that Escherichia coli is indeed able to measure its glycolytic flux and uses this signal for metabolic regulation. Combining experiment and theory, we show how this flux-sensing function could emerge from an aggregate of several molecular mechanisms: First, the system of reactions of lower glycolysis and the feedforward activation of fructose-1,6-bisphosphate on pyruvate kinase translate flux information into the concentration of the metabolite fructose-1,6-bisphosphate. The interaction of this "flux-signaling metabolite" with the transcription factor Cra then leads to flux-dependent regulation. By responding to glycolytic flux, rather than to the concentration of individual carbon sources, the cell may minimize sensing and regulatory expenses. |
| Volume | 110(3) |
| Pages | 1130-5 |
| Published | 2013-1-15 |
| DOI | 10.1073/pnas.1202582110 |
| PII | 1202582110 |
| PMID | 23277571 |
| PMC | PMC3549114 |
| MeSH | Escherichia coli K12 / genetics Escherichia coli K12 / metabolism* Feedback, Physiological Fructose-Bisphosphatase / metabolism Genes, Bacterial Glycolysis Kinetics Metabolic Networks and Pathways Models, Biological Pyruvate Kinase / genetics Pyruvate Kinase / metabolism Transcription, Genetic |
| IF | 9.412 |
| Times Cited | 108 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 8 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | ME9062(BW25113) JW1666-KC(pykF) |