Reference - Detail
| RRC ID | 35608 |
|---|---|
| Author | Peil L, Starosta AL, Lassak J, Atkinson GC, Virumäe K, Spitzer M, Tenson T, Jung K, Remme J, Wilson DN. |
| Title | Distinct XPPX sequence motifs induce ribosome stalling, which is rescued by the translation elongation factor EF-P. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
Ribosomes are the protein synthesizing factories of the cell, polymerizing polypeptide chains from their constituent amino acids. However, distinct combinations of amino acids, such as polyproline stretches, cannot be efficiently polymerized by ribosomes, leading to translational stalling. The stalled ribosomes are rescued by the translational elongation factor P (EF-P), which by stimulating peptide-bond formation allows translation to resume. Using metabolic stable isotope labeling and mass spectrometry, we demonstrate in vivo that EF-P is important for expression of not only polyproline-containing proteins, but also for specific subsets of proteins containing diprolyl motifs (XPP/PPX). Together with a systematic in vitro and in vivo analysis, we provide a distinct hierarchy of stalling triplets, ranging from strong stallers, such as PPP, DPP, and PPN to weak stallers, such as CPP, PPR, and PPH, all of which are substrates for EF-P. These findings provide mechanistic insight into how the characteristics of the specific amino acid substrates influence the fundamentals of peptide bond formation. |
| Volume | 110(38) |
| Pages | 15265-70 |
| Published | 2013-9-17 |
| DOI | 10.1073/pnas.1310642110 |
| PII | 1310642110 |
| PMID | 24003132 |
| PMC | PMC3780873 |
| MeSH | Amino Acid Motifs / genetics Chromatography, Liquid Escherichia coli K12 / metabolism Escherichia coli K12 / physiology* Humans Peptide Elongation Factors / metabolism* Proline / metabolism* Protein Biosynthesis / physiology* Proteomics Ribosomes / metabolism* Tandem Mass Spectrometry beta-Galactosidase |
| IF | 9.412 |
| Times Cited | 93 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | News |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | Keio collection |