論文 - 詳細
| RRC ID | 35615 |
|---|---|
| 著者 | Zeng J, Spiro S. |
| タイトル | Finely tuned regulation of the aromatic amine degradation pathway in Escherichia coli. |
| ジャーナル | J Bacteriol |
| Abstract |
FeaR is an AraC family regulator that activates transcription of the tynA and feaB genes in Escherichia coli. TynA is a periplasmic topaquinone- and copper-containing amine oxidase, and FeaB is a cytosolic NAD-linked aldehyde dehydrogenase. Phenylethylamine, tyramine, and dopamine are oxidized by TynA to the corresponding aldehydes, releasing one equivalent of H2O2 and NH3. The aldehydes can be oxidized to carboxylic acids by FeaB, and (in the case of phenylacetate) can be further degraded to enter central metabolism. Thus, phenylethylamine can be used as a carbon and nitrogen source, while tyramine and dopamine can be used only as sources of nitrogen. Using genetic, biochemical and computational approaches, we show that the FeaR binding site is a TGNCA-N8-AAA motif that occurs in 2 copies in the tynA and feaB promoters. We show that the coactivator for FeaR is the product rather than the substrate of the TynA reaction. The feaR gene is upregulated by carbon or nitrogen limitation, which we propose reflects regulation of feaR by the cyclic AMP receptor protein (CRP) and the nitrogen assimilation control protein (NAC), respectively. In carbon-limited cells grown in the presence of a TynA substrate, tynA and feaB are induced, whereas in nitrogen-limited cells, only the tynA promoter is induced. We propose that tynA and feaB expression is finely tuned to provide the FeaB activity that is required for carbon source utilization and the TynA activity required for nitrogen and carbon source utilization. |
| 巻・号 | 195(22) |
| ページ | 5141-50 |
| 公開日 | 2013-11-1 |
| DOI | 10.1128/JB.00837-13 |
| PII | JB.00837-13 |
| PMID | 24013633 |
| PMC | PMC3811602 |
| MeSH | Aldehyde Dehydrogenase / genetics Aldehyde Dehydrogenase / metabolism Aldehyde Oxidoreductases / genetics Aldehyde Oxidoreductases / metabolism* Amine Oxidase (Copper-Containing) / genetics Amine Oxidase (Copper-Containing) / metabolism Amines / metabolism* Binding Sites Biotransformation Carbon / metabolism DNA, Bacterial / metabolism Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial Hydrocarbons, Aromatic / metabolism* Metabolic Networks and Pathways* Monoamine Oxidase / genetics Monoamine Oxidase / metabolism* Nitrogen / metabolism Promoter Regions, Genetic Protein Binding Trans-Activators / genetics Trans-Activators / metabolism* Transcription Factors / genetics Transcription Factors / metabolism |
| IF | 3.006 |
| 引用数 | 13 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | JW3839-KC(glnG) JW1967-KC(nac) |