RRC ID 35618
Author Talluri S, Raj SM, Christopher LP.
Title Consolidated bioprocessing of untreated switchgrass to hydrogen by the extreme thermophile Caldicellulosiruptor saccharolyticus DSM 8903.
Journal Bioresour Technol
Abstract The abilities of the extreme thermophilic bacterium Caldicellulosiruptor saccharolyticus DSM 8903 to ferment switchgrass (SWG), microcrystalline cellulose (MCC) and glucose to hydrogen (H2) in one-step were examined. Hydrogen production from glucose reached the theoretical maximum for dark fermentation of 4 mol H2/mol glucose. The H2 yield on MCC and SWG after 6 days of fermentation was 23.2 mmol H2/L or 9.4 mmol H2/g MCC and 14.3 mmol H2/L or 11.2 mmol H2/g SWG, respectively. The rate of H2 formation however was higher on MCC (0.7 mmol/Lh) than SWG (0.1 mmol/Lh). C. saccharolyticus DSM 8903 was able to produce H2 directly from mechanically-comminuted SWG without any physicochemical or biological pretreatment. Combining four processing steps (pretreatment, enzyme production, saccharification and fermentation) into a single biorefinery operation makes C. saccharolyticus DSM 8903 a promising candidate for consolidated bioprocessing (CBP) of lignocellulosic biomass.
Volume 139
Pages 272-9
Published 2013-7-1
DOI 10.1016/j.biortech.2013.04.005
PII S0960-8524(13)00609-3
PMID 23665687
MeSH Anaerobiosis / drug effects Biotechnology / methods* Carbon / metabolism Carbon Dioxide / metabolism Cellulose / metabolism Glucose / pharmacology Gram-Positive Bacteria / drug effects Gram-Positive Bacteria / growth & development Gram-Positive Bacteria / metabolism* Gram-Positive Bacteria / ultrastructure Hydrogen / metabolism* Panicum / drug effects Panicum / metabolism* Polymers / pharmacology Temperature* Time Factors
IF 7.539
Times Cited 46
Prokaryotes E. coli