Reference - Detail
| RRC ID | 35625 |
|---|---|
| Author | Kozmin SG, Schaaper RM. |
| Title | Genetic characterization of moaB mutants of Escherichia coli. |
| Journal | Res Microbiol |
| Abstract |
The moaABCDE operon of Escherichia coli encodes enzymes essential for the biosynthesis of the molybdenum cofactor (Moco). However, the role of the moaB gene within this operon has remained enigmatic. Here, we have investigated the effect of moaB defects on two phenotypes diagnostic for Moco-deficiency: chlorate-resistance and sensitivity to the base analog 6-N-hydroxylaminopurine (HAP). We found that transposon insertions in moaB caused partial Moco-deficiency associated with chlorate-resistance, but not for HAP-sensitivity. On the other hand, in-frame deletions of moaB, or moaB overexpression, had no effect on either phenotype. Our combined data are consistent with the lack of any role for MoaB in Moco biosynthesis in E. coli. |
| Volume | 164(7) |
| Pages | 689-94 |
| Published | 2013-9-1 |
| DOI | 10.1016/j.resmic.2013.05.001 |
| PII | S0923-2508(13)00088-0 |
| PMID | 23680484 |
| PMC | PMC3743958 |
| MeSH | Adenine / analogs & derivatives* Adenine / pharmacology Chlorates / pharmacology* Coenzymes / biosynthesis Escherichia coli / drug effects* Escherichia coli / enzymology* Escherichia coli / genetics Escherichia coli / metabolism Escherichia coli Proteins / genetics* Escherichia coli Proteins / metabolism Metalloproteins / biosynthesis Molybdenum Cofactors Mutagenesis, Insertional Pteridines |
| IF | 3.217 |
| Times Cited | 3 |
| WOS Category | MICROBIOLOGY |
| Resource | |
| Prokaryotes E. coli | JD26193 JD26194 JD26195 JD26196 JD26197 JD26198 |