| RRC ID |
35627
|
| Author |
Ito K, Hamasaki K, Kayamori A, Nguyen PA, Amagai K, Wachi M.
|
| Title |
A secondary structure in the 5' untranslated region of adhE mRNA required for RNase G-dependent regulation.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
Escherichia coli RNase G is involved in the degradation of several mRNAs, including adhE and eno, which encode alcohol dehydrogenase and enolase respectively. Previous research indicates that the 5' untranslated region (5'-UTR) of adhE mRNA gives RNase G-dependency to lacZ mRNA when tagged at the 5'-end, but it has not been elucidated yet how RNase G recognizes adhE mRNA. Primer extension analysis revealed that RNase G cleaved a phosphodiester bond between -19A and -18C in the 5'-UTR (the A of the start codon was defined as +1). Site-directed mutagenesis indicated that RNase G did not recognize the nucleotides at -19 and -18. Random deletion analysis indicated that the sequence from -145 to -125 was required for RNase G-dependent degradation. Secondary structure prediction and further site-directed deletion suggested that the stem-loop structure, with a bubble in the stem, is required for RNaseG-dependent degradation of adhE mRNA.
|
| Volume |
77(12)
|
| Pages |
2473-9
|
| Published |
2013-1-1
|
| DOI |
10.1271/bbb.130618
|
| PII |
DN/JST.JSTAGE/bbb/130618
|
| PMID |
24317071
|
| MeSH |
5' Untranslated Regions / genetics*
Alcohol Dehydrogenase / genetics*
Aldehyde Oxidoreductases / genetics*
Base Sequence
Binding Sites
Endoribonucleases / metabolism*
Escherichia coli / enzymology
Escherichia coli / genetics
Escherichia coli Proteins / genetics*
Escherichia coli Proteins / metabolism*
Gene Expression Regulation, Bacterial
Lac Operon / genetics
Nucleic Acid Conformation*
Protein Binding
|
| IF |
1.516
|
| Times Cited |
6
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
CHEMISTRY, APPLIED
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Prokaryotes E. coli |
ME8927(CAG18570) |
