Reference - Detail
| RRC ID | 35627 |
|---|---|
| Author | Ito K, Hamasaki K, Kayamori A, Nguyen PA, Amagai K, Wachi M. |
| Title | A secondary structure in the 5' untranslated region of adhE mRNA required for RNase G-dependent regulation. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Escherichia coli RNase G is involved in the degradation of several mRNAs, including adhE and eno, which encode alcohol dehydrogenase and enolase respectively. Previous research indicates that the 5' untranslated region (5'-UTR) of adhE mRNA gives RNase G-dependency to lacZ mRNA when tagged at the 5'-end, but it has not been elucidated yet how RNase G recognizes adhE mRNA. Primer extension analysis revealed that RNase G cleaved a phosphodiester bond between -19A and -18C in the 5'-UTR (the A of the start codon was defined as +1). Site-directed mutagenesis indicated that RNase G did not recognize the nucleotides at -19 and -18. Random deletion analysis indicated that the sequence from -145 to -125 was required for RNase G-dependent degradation. Secondary structure prediction and further site-directed deletion suggested that the stem-loop structure, with a bubble in the stem, is required for RNaseG-dependent degradation of adhE mRNA. |
| Volume | 77(12) |
| Pages | 2473-9 |
| Published | 2013-1-1 |
| DOI | 10.1271/bbb.130618 |
| PII | DN/JST.JSTAGE/bbb/130618 |
| PMID | 24317071 |
| MeSH | 5' Untranslated Regions / genetics* Alcohol Dehydrogenase / genetics* Aldehyde Oxidoreductases / genetics* Base Sequence Binding Sites Endoribonucleases / metabolism* Escherichia coli / enzymology Escherichia coli / genetics Escherichia coli Proteins / genetics* Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial Lac Operon / genetics Nucleic Acid Conformation* Protein Binding |
| IF | 1.516 |
| Times Cited | 6 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | ME8927(CAG18570) |