| RRC ID |
35633
|
| 著者 |
Nagamitsu H, Murata M, Kosaka T, Kawaguchi J, Mori H, Yamada M.
|
| タイトル |
Crucial roles of MicA and RybB as vital factors for σ-dependent cell lysis in Escherichia coli long-term stationary phase.
|
| ジャーナル |
J Mol Microbiol Biotechnol
|
| Abstract |
σ(E)-dependent cell lysis has been proposed to eliminate damaged cells in the stationary phase in Escherichia coli. In order to explore the relationship of this process to long-term stationary phase existence, we considered that micA and rybB could be important small regulatory RNA (sRNA) genes for σ(E)-dependent cell lysis. A long-term stationary phase was observed at temperatures of <37°C, but not >38°C, and was found even in an rpoS knock-out background. Strains with disrupted micA or rybB were incapable of long-term stationary phase existence. Both strains drastically lost survivability accompanied by a dramatic accumulation of mutations. These findings allow us to speculate that σ(E)-dependent cell lysis plays a key role in the establishment of the long-term stationary phase, presumably by eliminating damaged cells and thus preventing the over-accumulation of mutations.
|
| 巻・号 |
23(3)
|
| ページ |
227-32
|
| 公開日 |
2013-1-1
|
| DOI |
10.1159/000350370
|
| PII |
000350370
|
| PMID |
23594456
|
| MeSH |
DNA Glycosylases / genetics
DNA Glycosylases / metabolism*
Escherichia coli / cytology*
Escherichia coli / metabolism*
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism*
Gene Expression Regulation, Bacterial
Sigma Factor / genetics
Sigma Factor / metabolism*
|
| IF |
0.803
|
| 引用数 |
9
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
|
