RRC ID |
35633
|
著者 |
Nagamitsu H, Murata M, Kosaka T, Kawaguchi J, Mori H, Yamada M.
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タイトル |
Crucial roles of MicA and RybB as vital factors for σ-dependent cell lysis in Escherichia coli long-term stationary phase.
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ジャーナル |
J Mol Microbiol Biotechnol
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Abstract |
σ(E)-dependent cell lysis has been proposed to eliminate damaged cells in the stationary phase in Escherichia coli. In order to explore the relationship of this process to long-term stationary phase existence, we considered that micA and rybB could be important small regulatory RNA (sRNA) genes for σ(E)-dependent cell lysis. A long-term stationary phase was observed at temperatures of <37°C, but not >38°C, and was found even in an rpoS knock-out background. Strains with disrupted micA or rybB were incapable of long-term stationary phase existence. Both strains drastically lost survivability accompanied by a dramatic accumulation of mutations. These findings allow us to speculate that σ(E)-dependent cell lysis plays a key role in the establishment of the long-term stationary phase, presumably by eliminating damaged cells and thus preventing the over-accumulation of mutations.
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巻・号 |
23(3)
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ページ |
227-32
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公開日 |
2013-1-1
|
DOI |
10.1159/000350370
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PII |
000350370
|
PMID |
23594456
|
MeSH |
DNA Glycosylases / genetics
DNA Glycosylases / metabolism*
Escherichia coli / cytology*
Escherichia coli / metabolism*
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism*
Gene Expression Regulation, Bacterial
Sigma Factor / genetics
Sigma Factor / metabolism*
|
IF |
0.803
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引用数 |
9
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WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
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リソース情報 |
原核生物(大腸菌) |
|