論文 - 詳細
| RRC ID | 35652 |
|---|---|
| 著者 | Soma Y, Tsuruno K, Wada M, Yokota A, Hanai T. |
| タイトル | Metabolic flux redirection from a central metabolic pathway toward a synthetic pathway using a metabolic toggle switch. |
| ジャーナル | Metab Eng |
| Abstract |
Overexpression of genes in production pathways and permanent knockout of genes in competing pathways are often employed to improve production titer and yield in metabolic engineering. However, the deletion of a pathway responsible for growth and cell maintenance has not previously been employed, even if its competition with the production pathway is obvious. In order to optimize intracellular metabolism at each fermentation phase for bacterial growth and production, a methodology employing conditional knockout is required. We constructed a metabolic toggle switch in Escherichia coli as a novel conditional knockout approach and applied it to isopropanol production. The resulting redirection of excess carbon flux caused by interruption of the TCA cycle via switching gltA OFF improved isopropanol production titer and yield up to 3.7 and 3.1 times, respectively. This approach is a useful tool to redirect carbon flux responsible for bacterial growth and/or cell maintenance toward a synthetic production pathway. |
| 巻・号 | 23 |
| ページ | 175-84 |
| 公開日 | 2014-5-1 |
| DOI | 10.1016/j.ymben.2014.02.008 |
| PII | S1096-7176(14)00030-5 |
| PMID | 24576819 |
| MeSH | 2-Propanol / metabolism* Citric Acid Cycle* Escherichia coli* / genetics Escherichia coli* / metabolism Gene Knockdown Techniques Metabolic Engineering / methods* |
| IF | 7.263 |
| 引用数 | 89 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 21 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | NA |