論文 - 詳細
| RRC ID | 35657 |
|---|---|
| 著者 | Winkler JD, Garcia C, Olson M, Callaway E, Kao KC. |
| タイトル | Evolved osmotolerant Escherichia coli mutants frequently exhibit defective N-acetylglucosamine catabolism and point mutations in cell shape-regulating protein MreB. |
| ジャーナル | Appl Environ Microbiol |
| Abstract |
Biocatalyst robustness toward stresses imposed during fermentation is important for efficient bio-based production. Osmotic stress, imposed by high osmolyte concentrations or dense populations, can significantly impact growth and productivity. In order to better understand the osmotic stress tolerance phenotype, we evolved sexual (capable of in situ DNA exchange) and asexual Escherichia coli strains under sodium chloride (NaCl) stress. All isolates had significantly improved growth under selection and could grow in up to 0.80 M (47 g/liter) NaCl, a concentration that completely inhibits the growth of the unevolved parental strains. Whole genome resequencing revealed frequent mutations in genes controlling N-acetylglucosamine catabolism (nagC, nagA), cell shape (mrdA, mreB), osmoprotectant uptake (proV), and motility (fimA). Possible epistatic interactions between nagC, nagA, fimA, and proV deletions were also detected when reconstructed as defined mutations. Biofilm formation under osmotic stress was found to be decreased in most mutant isolates, coupled with perturbations in indole secretion. Transcriptional analysis also revealed significant changes in ompACGL porin expression and increased transcription of sulfonate uptake systems in the evolved mutants. These findings expand our current knowledge of the osmotic stress phenotype and will be useful for the rational engineering of osmotic tolerance into industrial strains in the future. |
| 巻・号 | 80(12) |
| ページ | 3729-40 |
| 公開日 | 2014-6-1 |
| DOI | 10.1128/AEM.00499-14 |
| PII | AEM.00499-14 |
| PMID | 24727267 |
| PMC | PMC4054140 |
| MeSH | Acetylglucosamine / metabolism* Escherichia coli / chemistry Escherichia coli / cytology* Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / genetics* Escherichia coli Proteins / metabolism Osmotic Pressure* Point Mutation* Sodium Chloride / metabolism |
| IF | 4.016 |
| 引用数 | 31 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | JE6886(G1) JE6887(G2) JE5544(G3) JE5971(G5) JE5596(G6) JE5500(A5) |