RRC ID 3568
Author Chuang CF, Vanhoven MK, Fetter RD, Verselis VK, Bargmann CI.
Title An innexin-dependent cell network establishes left-right neuronal asymmetry in C. elegans.
Journal Cell
Abstract Gap junctions are widespread in immature neuronal circuits, but their functional significance is poorly understood. We show here that a transient network formed by the innexin gap-junction protein NSY-5 coordinates left-right asymmetry in the developing nervous system of Caenorhabditis elegans. nsy-5 is required for the left and right AWC olfactory neurons to establish stochastic, asymmetric patterns of gene expression during embryogenesis. nsy-5-dependent gap junctions in the embryo transiently connect the AWC cell bodies with those of numerous other neurons. Both AWCs and several other classes of nsy-5-expressing neurons participate in signaling that coordinates left-right AWC asymmetry. The right AWC can respond to nsy-5 directly, but the left AWC requires nsy-5 function in multiple cells of the network. NSY-5 forms hemichannels and intercellular gap-junction channels in Xenopus oocytes, consistent with a combination of cell-intrinsic and network functions. These results provide insight into gap-junction activity in developing circuits.
Volume 129(4)
Pages 787-99
Published 2007-5-18
DOI 10.1016/j.cell.2007.02.052
PII S0092-8674(07)00451-5
PMID 17512411
MeSH Amino Acid Sequence Animals Base Sequence Body Patterning / physiology* Caenorhabditis elegans / embryology* Caenorhabditis elegans / metabolism Caenorhabditis elegans / ultrastructure Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / isolation & purification Caenorhabditis elegans Proteins / metabolism* Calcium Signaling / physiology Cell Communication / physiology Cell Differentiation / physiology Connexins / genetics Connexins / isolation & purification Connexins / metabolism* Functional Laterality / physiology Gap Junctions / metabolism Membrane Proteins / metabolism Molecular Sequence Data Nerve Net / embryology* Nerve Net / metabolism Nerve Net / ultrastructure Nervous System / embryology* Nervous System / metabolism Nervous System / ultrastructure Neurons / metabolism* Neurons / ultrastructure Olfactory Pathways / embryology Olfactory Pathways / metabolism Olfactory Pathways / ultrastructure
IF 36.216
Times Cited 70
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
C.elegans tm1896