RRC ID 35689
Author Koyama-Nasu R, Takahashi R, Yanagida S, Nasu-Nishimura Y, Oyama M, Kozuka-Hata H, Haruta R, Manabe E, Hoshino-Okubo A, Omi H, Yanaihara N, Okamoto A, Tanaka T, Akiyama T.
Title The cancer stem cell marker CD133 interacts with plakoglobin and controls desmoglein-2 protein levels.
Journal PLoS ONE
Abstract The pentaspan membrane glycoprotein CD133 (also known as prominin-1) has been widely used as a marker for both cancer and normal stem cells. However, the function of CD133 has not been elucidated. Here we describe a cancer stem cell line established from clear cell carcinoma of the ovary (CCC) and show that CD133 interacts with plakoglobin (also known as γ-catenin), a desmosomal linker protein. We further demonstrate that knockdown of CD133 by RNA interference (RNAi) results in the downregulation of desmoglein-2, a desmosomal cadherin, and abrogates cell-cell adhesion and tumorigenicity of CCC stem cells. We speculate that CD133 may be a promising target for cancer chemotherapy.
Volume 8(1)
Pages e53710
Published 2013
DOI 10.1371/journal.pone.0053710
PII PONE-D-12-29013
PMID 23326490
PMC PMC3542344
MeSH AC133 Antigen Animals Antigens, CD / metabolism* Biomarkers, Tumor / metabolism* Caco-2 Cells Cell Adhesion Cell Transformation, Neoplastic / metabolism Cell Transformation, Neoplastic / pathology Desmoglein 2 / metabolism* Glycoproteins / metabolism* Humans Mice Neoplastic Stem Cells / metabolism* Neoplastic Stem Cells / pathology Peptides / metabolism* Protein Binding gamma Catenin / metabolism*
IF 2.766
Times Cited 5
WOS Category MULTIDISCIPLINARY SCIENCES
Resource
DNA material CS-RfA-CG (RDB04390) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393).