| RRC ID |
35710
|
| Author |
Ujino S, Nishitsuji H, Hishiki T, Sugiyama K, Takaku H, Shimotohno K.
|
| Title |
Hepatitis C virus utilizes VLDLR as a novel entry pathway.
|
| Journal |
Proc Natl Acad Sci U S A
|
| Abstract |
Various host factors are involved in the cellular entry of hepatitis C virus (HCV). In addition to the factors previously reported, we discovered that the very-low-density lipoprotein receptor (VLDLR) mediates HCV entry independent of CD81. Culturing Huh7.5 cells under hypoxic conditions significantly increased HCV entry as a result of the expression of VLDLR, which was not expressed under normoxic conditions in this cell line. Ectopic VLDLR expression conferred susceptibility to HCV entry of CD81-deficient Huh7.5 cells. Additionally, VLDLR-mediated HCV entry was not affected by the knockdown of cellular factors known to act as HCV receptors or HCV entry factors. Because VLDLR is expressed in primary human hepatocytes, our results suggest that VLDLR functions in vivo as an HCV receptor independent of canonical CD81-mediated HCV entry.
|
| Volume |
113(1)
|
| Pages |
188-93
|
| Published |
2016-1-5
|
| DOI |
10.1073/pnas.1506524113
|
| PII |
1506524113
|
| PMID |
26699506
|
| PMC |
PMC4711846
|
| MeSH |
Anaerobiosis
Animals
Cell Line
Hepacivirus / physiology*
Hepatitis C / virology*
Hepatocytes / virology*
Humans
Lipoproteins, VLDL / metabolism
Mice
Mice, Transgenic
Occludin / genetics
Receptors, LDL / genetics
Receptors, LDL / physiology*
Receptors, Virus / genetics
Receptors, Virus / physiology*
Tetraspanin 28 / genetics
Tetraspanin 28 / physiology
Virus Internalization*
|
| IF |
9.412
|
| Times Cited |
27
|
|
WOS Category
|
VIROLOGY
|
| Resource |
| DNA material |
CSII-CMV-MCS (RDB04377)
pRSV-Rev (RDB08121)
pCAG-HIVgp (RDB04394)
pCMV-VSV-G (RDB04392) |
| Human and Animal Cells |
Hepa 1-6(RCB1638) |
