RRC ID 35719
Author Shibata T, Maki K, Hadano J, Fujikawa T, Kitazaki K, Koshiba T, Kawabata S.
Title Crosslinking of a Peritrophic Matrix Protein Protects Gut Epithelia from Bacterial Exotoxins.
Journal PLoS Pathog.
Abstract Transglutaminase (TG) catalyzes protein-protein crosslinking, which has important and diverse roles in vertebrates and invertebrates. Here we demonstrate that Drosophila TG crosslinks drosocrystallin, a peritrophic matrix protein, to form a stable fiber structure on the gut peritrophic matrix. RNA interference (RNAi) of the TG gene was highly lethal in flies and induced apoptosis of gut epithelial cells after oral infection with Pseudomonas entomophila. Moreover, AprA, a metalloprotease secreted by P. entomophila, digested non-crosslinked drosocrystallin fibers, but not drosocrystallin fibers crosslinked by TG. In vitro experiments using recombinant drosocrystallin and monalysin proteins demonstrated that monalysin, a pore-forming exotoxin of P. entomophila, was adsorbed on the crosslinked drosocrystallin fibers in the presence of P. entomophila culture supernatant. In addition, gut-specific TG-RNAi flies had a shorter lifespan than control flies after ingesting P. entomophila, whereas the lifespan after ingesting AprA-knockout P. entomophila was at control levels. We conclude that drosocrystallin fibers crosslinked by TG, but not non-crosslinked drosocrystallin fibers, form an important physical barrier against exotoxins of invading pathogenic microbes.
Volume 11(10)
Pages e1005244
Published 2015-10
DOI 10.1371/journal.ppat.1005244
PII PPATHOGENS-D-15-01211
PMID 26506243
PMC PMC4646701
MeSH Animals Bacterial Toxins / toxicity* Calcium / metabolism Drosophila Drosophila Proteins / chemistry Drosophila Proteins / physiology* Eye Proteins / chemistry Eye Proteins / physiology* Intestinal Mucosa / microbiology* Pseudomonas / pathogenicity Transglutaminases / physiology
IF 6.463
Times Cited 20
WOS Category PARASITOLOGY MICROBIOLOGY VIROLOGY
Resource
Drosophila 7356R-1 7356R-2