| RRC ID |
35723
|
| 著者 |
Koba C, Haruta M, Matsunaga Y, Matsumura K, Haga E, Sasaki Y, Ikeda T, Takamatsu K, Nishimura Y, Senju S.
|
| タイトル |
Therapeutic effect of human iPS-cell-derived myeloid cells expressing IFN-β against peritoneally disseminated cancer in xenograft models.
|
| ジャーナル |
PLoS One
|
| Abstract |
We recently developed a method to generate myeloid cells with proliferation capacity from human iPS cells. iPS-ML (iPS-cell-derived myeloid/macrophage line), generated by introducing proliferation and anti-senescence factors into iPS-cell-derived myeloid cells, grew continuously in an M-CSF-dependent manner. A large number of cells exhibiting macrophage-like properties can be readily obtained by using this technology. In the current study, we evaluated the possible application of iPS-ML in anti-cancer therapy. We established a model of peritoneally disseminated gastric cancer by intraperitoneally injecting NUGC-4 human gastric cancer cells into SCID mice. When iPS-ML were injected intraperitoneally into the mice with pre-established peritoneal NUGC-4 tumors, iPS-ML massively accumulated and infiltrated into the tumor tissues. iPS-ML expressing IFN-β (iPS-ML/IFN-β) significantly inhibited the intra-peritoneal growth of NUGC-4 cancer. Furthermore, iPS-ML/IFN-β also inhibited the growth of human pancreatic cancer MIAPaCa-2 in a similar model. iPS-ML are therefore a promising treatment agent for peritoneally disseminated cancers, for which no standard treatment is currently available.
|
| 巻・号 |
8(6)
|
| ページ |
e67567
|
| 公開日 |
2013-1-1
|
| DOI |
10.1371/journal.pone.0067567
|
| PII |
PONE-D-12-29947
|
| PMID |
23826321
|
| PMC |
PMC3691167
|
| MeSH |
Animals
Cell Line, Tumor
Cell Proliferation
Cytokines / pharmacology
Humans
Induced Pluripotent Stem Cells / cytology*
Injections, Intraperitoneal
Interferon-beta / metabolism*
Macrophages / pathology
Mice
Mice, SCID
Myeloid Cells / metabolism*
Myeloid Cells / transplantation*
Pancreatic Neoplasms / pathology
Pancreatic Neoplasms / therapy
Peritoneal Neoplasms / secondary
Peritoneal Neoplasms / therapy*
Peritoneum / metabolism
Peritoneum / pathology
Receptor, ErbB-2 / metabolism
Single-Chain Antibodies / metabolism
Stem Cell Transplantation*
Xenograft Model Antitumor Assays*
|
| IF |
2.74
|
| 引用数 |
22
|
|
WOS 分野
|
IMMUNOLOGY
|
| リソース情報 |
| 遺伝子材料 |
pCMV-VSV-G-RSV-Rev (RDB04393)
pCAG-HIVgp (RDB04394). |
