RRC ID 35738
Author Takemoto Y, Furuta M, Li XK, Strong-Sparks WJ, Hashimoto Y.
Title LckBP1, a proline-rich protein expressed in haematopoietic lineage cells, directly associates with the SH3 domain of protein tyrosine kinase p56lck.
Journal EMBO J.
Abstract The Lck tyrosine kinase molecule plays an essential role in T cell activation and T cell development. Using the expression cloning technique, we have isolated a gene that encodes a molecule, LckBP1, able to associate with murine Lck. Analysis of full-length LckBP1 cDNA indicates at least four potentially important segments: a four tandem 37 amino acid repeat motif with a potential helix-turn-helix DNA binding motif; a proline-rich region; a proline-glutamate repeat; and an SH3 domain. These four regions are very similar to the human haematopoietic-specific protein 1 (HS1). Deletion mutant analysis of LckBP1 revealed two proline-rich regions that permit association with Lck SH3. One region contains prolines conserved among HS1 and cortactin, and the other region contains a potential MAP kinase recognition site. In vivo association between Lck and LckBP1 was confirmed by immunoprecipitation of lysates from a pre-T cell line and adult thymocytes using antibodies specific for Lck and LckBP1. LckBP1 is tyrosine phosphorylated after T-cell receptor stimulation. The SH3 domain and the potential helix-turn-helix motif in LckBP1 suggest that this molecule may associate with various molecules and function as a DNA binding molecule. The data also suggest that LckBP1 mediates intracellular signalling through Lck in T cells.
Volume 14(14)
Pages 3403-14
Published 1995-7-17
PMID 7628441
PMC PMC394407
MeSH 3T3 Cells Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals Base Sequence Binding Sites Cell Line Cloning, Molecular DNA, Complementary DNA-Binding Proteins / chemistry DNA-Binding Proteins / metabolism* Hematopoietic Stem Cells / metabolism* Humans Lymphocyte Specific Protein Tyrosine Kinase p56(lck) Mice Mice, Inbred BALB C Molecular Sequence Data Peptides / metabolism Proline / metabolism* Proline-Rich Protein Domains Protein Conformation Protein-Tyrosine Kinases / metabolism* Receptors, Antigen, T-Cell / metabolism Sequence Homology, Amino Acid Signal Transduction T-Lymphocytes / metabolism
IF 10.557
Times Cited 80
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
DNA material LCK (RDB01789) Mouse HS1 (RDB01790) GST-Lck SH3 (RDB02115) GST-Lck SH2 (RDB02116) GST-Lck SH32 (RDB02117) GST-Lck Kinase (RDB02118) GST-HS1-#1 (RDB02123) GST-HS1-#2 (RDB02124) GST-HS1-#3 (RDB02125) GST-HS1-#4 (RDB02126) GST-HS1-#5 (RDB02127) GST-HS1-#6 (RDB02128) GST-HS1-#7 (RDB02129) GST-HS1-#8 (RDB02130) GST-HS1-P1 (RDB02131) GST-HS1-P2 (RDB02132) GST-HS1-P3 (RDB02133) GST-HS1-P4 (RDB02134) KKF cDNA library (RDB02148).