| RRC ID |
35738
|
| Author |
Takemoto Y, Furuta M, Li XK, Strong-Sparks WJ, Hashimoto Y.
|
| Title |
LckBP1, a proline-rich protein expressed in haematopoietic lineage cells, directly associates with the SH3 domain of protein tyrosine kinase p56lck.
|
| Journal |
EMBO J
|
| Abstract |
The Lck tyrosine kinase molecule plays an essential role in T cell activation and T cell development. Using the expression cloning technique, we have isolated a gene that encodes a molecule, LckBP1, able to associate with murine Lck. Analysis of full-length LckBP1 cDNA indicates at least four potentially important segments: a four tandem 37 amino acid repeat motif with a potential helix-turn-helix DNA binding motif; a proline-rich region; a proline-glutamate repeat; and an SH3 domain. These four regions are very similar to the human haematopoietic-specific protein 1 (HS1). Deletion mutant analysis of LckBP1 revealed two proline-rich regions that permit association with Lck SH3. One region contains prolines conserved among HS1 and cortactin, and the other region contains a potential MAP kinase recognition site. In vivo association between Lck and LckBP1 was confirmed by immunoprecipitation of lysates from a pre-T cell line and adult thymocytes using antibodies specific for Lck and LckBP1. LckBP1 is tyrosine phosphorylated after T-cell receptor stimulation. The SH3 domain and the potential helix-turn-helix motif in LckBP1 suggest that this molecule may associate with various molecules and function as a DNA binding molecule. The data also suggest that LckBP1 mediates intracellular signalling through Lck in T cells.
|
| Volume |
14(14)
|
| Pages |
3403-14
|
| Published |
1995-7-17
|
| DOI |
10.1002/j.1460-2075.1995.tb07346.x
|
| PMID |
7628441
|
| PMC |
PMC394407
|
| MeSH |
3T3 Cells
Adaptor Proteins, Signal Transducing
Amino Acid Sequence
Animals
Base Sequence
Binding Sites
Cell Line
Cloning, Molecular
DNA, Complementary
DNA-Binding Proteins / chemistry
DNA-Binding Proteins / metabolism*
Hematopoietic Stem Cells / metabolism*
Humans
Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Peptides / metabolism
Proline / metabolism*
Proline-Rich Protein Domains
Protein Conformation
Protein-Tyrosine Kinases / metabolism*
Receptors, Antigen, T-Cell / metabolism
Sequence Homology, Amino Acid
Signal Transduction
T-Lymphocytes / metabolism
|
| IF |
9.889
|
| Times Cited |
82
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| DNA material |
LCK (RDB01789)
Mouse HS1 (RDB01790)
GST-Lck SH3 (RDB02115)
GST-Lck SH2 (RDB02116)
GST-Lck SH32 (RDB02117)
GST-Lck Kinase (RDB02118)
GST-HS1-#1 (RDB02123)
GST-HS1-#2 (RDB02124)
GST-HS1-#3 (RDB02125)
GST-HS1-#4 (RDB02126)
GST-HS1-#5 (RDB02127)
GST-HS1-#6 (RDB02128)
GST-HS1-#7 (RDB02129)
GST-HS1-#8 (RDB02130)
GST-HS1-P1 (RDB02131)
GST-HS1-P2 (RDB02132)
GST-HS1-P3 (RDB02133)
GST-HS1-P4 (RDB02134)
KKF cDNA library (RDB02148). |
| Human and Animal Cells |
BALB/3T3 clone A31(RCB0005) |
