RRC ID 35738
Author Takemoto Y, Furuta M, Li XK, Strong-Sparks WJ, Hashimoto Y.
Title LckBP1, a proline-rich protein expressed in haematopoietic lineage cells, directly associates with the SH3 domain of protein tyrosine kinase p56lck.
Journal EMBO J
Abstract The Lck tyrosine kinase molecule plays an essential role in T cell activation and T cell development. Using the expression cloning technique, we have isolated a gene that encodes a molecule, LckBP1, able to associate with murine Lck. Analysis of full-length LckBP1 cDNA indicates at least four potentially important segments: a four tandem 37 amino acid repeat motif with a potential helix-turn-helix DNA binding motif; a proline-rich region; a proline-glutamate repeat; and an SH3 domain. These four regions are very similar to the human haematopoietic-specific protein 1 (HS1). Deletion mutant analysis of LckBP1 revealed two proline-rich regions that permit association with Lck SH3. One region contains prolines conserved among HS1 and cortactin, and the other region contains a potential MAP kinase recognition site. In vivo association between Lck and LckBP1 was confirmed by immunoprecipitation of lysates from a pre-T cell line and adult thymocytes using antibodies specific for Lck and LckBP1. LckBP1 is tyrosine phosphorylated after T-cell receptor stimulation. The SH3 domain and the potential helix-turn-helix motif in LckBP1 suggest that this molecule may associate with various molecules and function as a DNA binding molecule. The data also suggest that LckBP1 mediates intracellular signalling through Lck in T cells.
Volume 14(14)
Pages 3403-14
Published 1995-7-17
DOI 10.1002/j.1460-2075.1995.tb07346.x
PMID 7628441
PMC PMC394407
MeSH 3T3 Cells Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals Base Sequence Binding Sites Cell Line Cloning, Molecular DNA, Complementary DNA-Binding Proteins / chemistry DNA-Binding Proteins / metabolism* Hematopoietic Stem Cells / metabolism* Humans Lymphocyte Specific Protein Tyrosine Kinase p56(lck) Mice Mice, Inbred BALB C Molecular Sequence Data Peptides / metabolism Proline / metabolism* Proline-Rich Protein Domains Protein Conformation Protein-Tyrosine Kinases / metabolism* Receptors, Antigen, T-Cell / metabolism Sequence Homology, Amino Acid Signal Transduction T-Lymphocytes / metabolism
IF 9.889
Times Cited 82
DNA material LCK (RDB01789) Mouse HS1 (RDB01790) GST-Lck SH3 (RDB02115) GST-Lck SH2 (RDB02116) GST-Lck SH32 (RDB02117) GST-Lck Kinase (RDB02118) GST-HS1-#1 (RDB02123) GST-HS1-#2 (RDB02124) GST-HS1-#3 (RDB02125) GST-HS1-#4 (RDB02126) GST-HS1-#5 (RDB02127) GST-HS1-#6 (RDB02128) GST-HS1-#7 (RDB02129) GST-HS1-#8 (RDB02130) GST-HS1-P1 (RDB02131) GST-HS1-P2 (RDB02132) GST-HS1-P3 (RDB02133) GST-HS1-P4 (RDB02134) KKF cDNA library (RDB02148).
Human and Animal Cells BALB/3T3 clone A31(RCB0005)