論文 - 詳細
| RRC ID | 35767 |
|---|---|
| 著者 | Kitagawa R, Mitsuki H, Okazaki T, Ogawa T. |
| タイトル | A novel DnaA protein-binding site at 94.7 min on the Escherichia coli chromosome. |
| ジャーナル | Mol Microbiol |
| Abstract |
There is a DNA sequence which has unusually high affinity for the DnaA protein of Escherichia coli between the glyV and amiB-mutL operons at 94.7 min on the genetic map. Affinity of DnaA protein for DNA was measured in vivo as the activity of beta-galactosidase produced from the lacZ gene on a plasmid fused to the 5'-terminal portion of the mioC gene, which is under the control of the DnaA protein. The chromosomal DNA segment between the two operons, carried on a compatible plasmid, derepressed the beta-galactosidase activity by titrating DnaA protein. Derepression occurred on the chromosomal dnaA gene as well, since it is autoregulated. Hence, as measured by immunoassays, one plasmid molecule carrying the DnaA-binding region titrated 370 dnaA molecules, which is a value eightfold higher than that for a plasmid containing the oriC region. We estimate that about 60% of the total cellular DnaA molecules are bound to this site. Four DnaA-binding sequences (DnaA boxes) and a DnaA-regulated promoter directing transcription of two small genes were present within a 250 bp stretch in this region but additional long DNA regions, including the fifth DnaA box located about 650 bp downstream, were required for maximum binding. A role for the DnaA-binding site in controlling DnaA-protein concentration in the cell cycle is discussed. |
| 巻・号 | 19(5) |
| ページ | 1137-47 |
| 公開日 | 1996-3-1 |
| DOI | 10.1046/j.1365-2958.1996.453983.x |
| PMID | 8830270 |
| MeSH | Bacterial Proteins / genetics Bacterial Proteins / metabolism* Base Sequence Binding Sites Chromosome Mapping Chromosomes, Bacterial* Cloning, Molecular DNA, Bacterial / metabolism* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Escherichia coli / genetics* Gene Expression Molecular Sequence Data Open Reading Frames Promoter Regions, Genetic Repressor Proteins / metabolism |
| IF | 3.418 |
| 引用数 | 99 |
| WOS 分野 | MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | anti-DnaA |