RRC ID 35792
Author Hiraoka K, Hayashi T, Kaneko R, Nasu-Nishimura Y, Koyama-Nasu R, Kawasaki Y, Akiyama T.
Title SOX9-mediated upregulation of LGR5 is important for glioblastoma tumorigenicity.
Journal Biochem. Biophys. Res. Commun.
Abstract LGR5 plays an important role in the self-renewal of stem cells and is used as a marker identifying self-renewing stem cells in small intestine and hair follicles. Moreover, LGR5 has been reported to be overexpressed in several cancers. SOX9 is a transcription factor that plays a key role in development, differentiation and lineage commitment in various tissues. It has also been reported that SOX9 is overexpressed in a variety of cancers and contributes to their malignant phenotype. Here we show that LGR5 is required for the tumorigenicity of glioblastoma cells. We further show that SOX9 is upregulated in glioblastoma cells and directly enhances the expression of LGR5. We also demonstrate that knockdown of SOX9 suppresses the proliferation and tumorigenicity of glioblastoma cells. These results suggest that SOX9-mediated transcriptional regulation of LGR5 is critical for the tumorigenicity of glioblastoma cells. We speculate that the SOX9-LGR5 pathway could be a potentially promising target for the therapy of glioblastoma.
Volume 460(2)
Pages 216-21
Published 2015-5-1
DOI 10.1016/j.bbrc.2015.03.012
PII S0006-291X(15)00437-4
PMID 25770425
MeSH Brain Neoplasms / metabolism* Brain Neoplasms / pathology Cell Line, Tumor Gene Knockdown Techniques Glioblastoma / metabolism* Glioblastoma / pathology Humans Receptors, G-Protein-Coupled / genetics Receptors, G-Protein-Coupled / physiology* SOX9 Transcription Factor / genetics SOX9 Transcription Factor / physiology* Up-Regulation / physiology*
IF 2.559
Times Cited 2
WOS Category BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
DNA material CS-RfA-CG (RDB04390) pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394).