Reference - Detail
| RRC ID | 35793 |
|---|---|
| Author | Katsuhara A, Fujiki F, Aoyama N, Tanii S, Morimoto S, Oka Y, Tsuboi A, Nakajima H, Kondo K, Tatsumi N, Nakata J, Nakae Y, Takashima S, Nishida S, Hosen N, Sogo S, Oji Y, Sugiyama H. |
| Title | Transduction of a novel HLA-DRB1*04:05-restricted, WT1-specific TCR gene into human CD4+ T cells confers killing activity against human leukemia cells. |
| Journal | Anticancer Res |
| Abstract |
BACKGROUND/AIM:Wilms' tumor gene 1 (WT1) product is a pan-tumor-associated antigen. We previously identified WT1 protein-derived promiscuous helper peptide, WT1332. Therefore, isolation and characterization of the WT1332-specific T-cell receptors (TCRs) are useful to develop broadly applicable TCR gene-based adoptive immunotherapy. MATERIALS AND METHODS:A novel HLA-DRB1*04:05-restricted WT1332-specific TCR gene was cloned and transduced into human CD4+ T-cells by using a lentiviral vector. RESULTS:The WT1332-specific TCR-transduced CD4+ T-cells showed strong proliferation and Th1-cytokine production in an HLA-DRB1*04:05-restricted, WT1332-specific manner. Furthermore, the WT1332-specific TCR-transduced CD4+ T-cells could lyse HLA-DRB1*04:05-positive, WT1-expressing leukemia cells in vitro. CONCLUSION:The novel TCR gene cloned here should be a promising tool to develop adoptive immunotherapy of WT1332-specific TCR-transduced CD4+ T-cells for the treatment of WT1-expressing cancer, such as leukemia. |
| Volume | 35(3) |
| Pages | 1251-61 |
| Published | 2015-3-1 |
| PII | 35/3/1251 |
| PMID | 25750272 |
| MeSH | Amino Acid Sequence CD4-Positive T-Lymphocytes / immunology* Cloning, Molecular Cytokines / analysis Cytotoxicity, Immunologic Genes, T-Cell Receptor* HLA-DRB1 Chains / immunology* Humans Immunotherapy, Adoptive* Jurkat Cells Leukemia / therapy* Molecular Sequence Data Transduction, Genetic WT1 Proteins / immunology* |
| IF | 1.994 |
| Times Cited | 5 |
| WOS Category | ONCOLOGY |
| Resource | |
| DNA material | CSII-EF-MCS-IRES2-Venus (RDB04384) pCMV-VSV-G-RSV-Rev (RDB04393 pCAG-HIVgp (RDB04394). |