RRC ID 35922
Author Nozaki H, Matsuzaki R, Yamamoto K, Kawachi M, Takahashi F.
Title Delineating a New Heterothallic Species of Volvox (Volvocaceae, Chlorophyceae) Using New Strains of "Volvox africanus".
Journal PLoS ONE
Abstract The volvocine algae represent an excellent model lineage in which to study evolution of female and male genders based on comparative analyses of related species. Among these species, Volvox carteri has been extensively studied as a model of an oogamous and complex organism. However, it may have unique derived features that are not present in other species of Volvox. Therefore, information regarding the characteristics of sexual reproduction of other species of Volvox is also important. In 1971, Starr studied four types of sexuality in several global strains identified as Volvox africanus; however, further taxonomic studies of these strains have been lacking, and strains of three of the four sexual types are not available. Here, we studied the morphology, sexual reproduction, and taxonomy of two V. africanus-like species isolated recently from Lake Biwa, Japan. These two species were very similar to two sexual types described by Starr in 1971: one producing dioecious sexual spheroids in heterothallic strains and the other forming both male spheroids and monoecious spheroids in a single strain. The former species produced zygotes with a reticulate cell wall, whereas a smooth zygote wall was observed in the latter species as in V. africanus previously reported from various localities around the world. Our multigene phylogenetic analysis demonstrated that these are sister species to each other. However, the presence of a compensatory base change in the most conserved region of the secondary structure of nuclear ribosomal DNA internal transcribed spacer-2, hybrid inviability demonstrated by intercrossing experiments, and morphological differences in the density of abutment between the gelatinous material of adjacent cells (individual sheaths) in the spheroid supported the recognition of the two species, V. africanus having a smooth zygote wall and V. reticuliferus Nozaki sp. nov. having a reticulate zygote wall.
Volume 10(11)
Pages e0142632
Published 2015
DOI 10.1371/journal.pone.0142632
PII PONE-D-15-38757
PMID 26562165
PMC PMC4643018
MeSH Base Sequence Classification / methods* DNA, Algal / chemistry DNA, Algal / genetics* DNA, Ribosomal Spacer / chemistry DNA, Ribosomal Spacer / genetics* Microscopy, Electron, Transmission Microscopy, Interference Molecular Sequence Data Nucleic Acid Conformation Phylogeny Reproduction / genetics Sequence Homology, Nucleic Acid Species Specificity Volvox / classification Volvox / genetics* Volvox / ultrastructure
IF 2.766
Times Cited 4
WOS Category PLANT SCIENCES
Resource
Algae NIES-3780 NIES-3781 NIES-3782 NIES-3783 NIES-3784 NIES-3785 NIES-3786