論文 - 詳細
| RRC ID | 35935 |
|---|---|
| 著者 | Yoshimoto N, Ikeda Y, Tatematsu K, Iijima M, Nakai T, Okajima T, Tanizawa K, Kuroda S. |
| タイトル | Cytokine-dependent activation of JAK-STAT pathway in Saccharomyces cerevisiae. |
| ジャーナル | Biotechnol Bioeng |
| Abstract |
Protein phosphorylation is an important post-translational modification for intracellular signaling molecules, mostly found in serine and threonine residues. Tyrosine phosphorylations are very few events (less than 0.1% to phosphorylated serine/threonine residues), but capable of governing cell fate decisions involved in proliferation, differentiation, apoptosis, and oncogenic transformation. Hence, it is important for drug discovery and system biology to measure the intracellular level of phosphotyrosine. Although mammalian cells have been conventionally utilized for this purpose, accurate determination of phosphotyrosine level often suffers from high background due to the unexpected crosstalk among endogenous signaling molecules. This situation led us firstly to establish the ligand-induced activation of homomeric receptor tyrosine kinase (i.e., epidermal growth factor receptor) in Saccharomyces cerevisiae, a lower eukaryote possessing organelles similar to higher eukaryote but not showing substantial level of tyrosine kinase activity. In this study, we expressed heteromeric receptor tyrosine kinase (i.e., a complex of interleukin-5 receptor (IL5R) α chain, common β chain, and JAK2 tyrosine kinase) in yeast. When coexpressed with a cell wall-anchored form of IL5, the yeast exerted the autophosphorylation of JAK2, followed by the phosphorylation of transcription factor STAT5a and subsequent nuclear accumulation of phosphorylated STAT5a. Taken together, yeast could be an ideal host for sensitive detection of phosphotyrosine generated by a wide variety of tyrosine kinases. Biotechnol. Bioeng. 2016;113: 1796-1804. © 2016 Wiley Periodicals, Inc. |
| 巻・号 | 113(8) |
| ページ | 1796-804 |
| 公開日 | 2016-8-1 |
| DOI | 10.1002/bit.25948 |
| PMID | 26853220 |
| MeSH | Cell Surface Display Techniques Cytokines / metabolism* Janus Kinases / metabolism* Phosphorylation Phosphotyrosine / metabolism Protein-Tyrosine Kinases / metabolism STAT Transcription Factors / metabolism* Saccharomyces cerevisiae / metabolism* Signal Transduction |
| IF | 4.002 |
| 引用数 | 2 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pGMH20 (RDB01956) pGMT20 (RDB01960) pGML10 (RDB01957). |