論文 - 詳細
| RRC ID | 35950 |
|---|---|
| 著者 | Izumi Y, Motoishi M, Furuse K, Furuse M. |
| タイトル | A tetraspanin regulates septate junction formation in Drosophila midgut. |
| ジャーナル | J Cell Sci |
| Abstract |
Septate junctions (SJs) are membrane specializations that restrict the free diffusion of solutes through the paracellular pathway in invertebrate epithelia. In arthropods, two morphologically different types of septate junctions are observed; pleated (pSJs) and smooth (sSJs), which are present in ectodermally and endodermally derived epithelia, respectively. Recent identification of sSJ-specific proteins, Mesh and Ssk, in Drosophila indicates that the molecular compositions of sSJs and pSJs differ. A deficiency screen based on immunolocalization of Mesh identified a tetraspanin family protein, Tsp2A, as a newly discovered protein involved in sSJ formation in Drosophila Tsp2A specifically localizes at sSJs in the midgut and Malpighian tubules. Compromised Tsp2A expression caused by RNAi or the CRISPR/Cas9 system was associated with defects in the ultrastructure of sSJs, changed localization of other sSJ proteins, and impaired barrier function of the midgut. In most Tsp2A mutant cells, Mesh failed to localize to sSJs and was distributed through the cytoplasm. Tsp2A forms a complex with Mesh and Ssk and these proteins are mutually interdependent for their localization. These observations suggest that Tsp2A cooperates with Mesh and Ssk to organize sSJs. |
| 巻・号 | 129(6) |
| ページ | 1155-64 |
| 公開日 | 2016-3-15 |
| DOI | 10.1242/jcs.180448 |
| PII | jcs.180448 |
| PMID | 26848177 |
| MeSH | Animals Digestive System / embryology Digestive System / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / embryology Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Female Male Membrane Proteins / genetics Membrane Proteins / metabolism Protein Binding Protein Transport Tetraspanins / genetics Tetraspanins / metabolism Tight Junctions / genetics Tight Junctions / metabolism* |
| IF | 4.573 |
| 引用数 | 19 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 11415R-2 |