RRC ID 35952
Author Vissers JH, Manning SA, Kulkarni A, Harvey KF.
Title A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth.
Journal Nat Commun
Abstract Libraries of transgenic Drosophila melanogaster carrying RNA interference (RNAi) constructs have been used extensively to perform large-scale functional genetic screens in vivo. For example, RNAi screens have facilitated the discovery of multiple components of the Hippo pathway, an evolutionarily conserved growth-regulatory network. Here we investigate an important technical limitation with the widely used VDRC KK RNAi collection. We find that approximately 25% of VDRC KK RNAi lines cause false-positive enhancement of the Hippo pathway, owing to ectopic expression of the Tiptop transcription factor. Of relevance to the broader Drosophila community, ectopic tiptop (tio) expression can also cause organ malformations and mask phenotypes such as organ overgrowth. To enhance the use of the VDRC KK RNAi library, we have generated a D. melanogaster strain that will allow researchers to test, in a single cross, whether their genetic screen of interest will be affected by ectopic tio expression.
Volume 7
Pages 10368
Published 2016-1-13
DOI 10.1038/ncomms10368
PII ncomms10368
PMID 26758424
PMC PMC4735554
MeSH Animals Animals, Genetically Modified* Drosophila Proteins / metabolism* Drosophila melanogaster / genetics* Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism Ectopic Gene Expression Female Intracellular Signaling Peptides and Proteins / metabolism* Male Nuclear Proteins / metabolism Protein Serine-Threonine Kinases / metabolism* RNA Interference* Trans-Activators / metabolism Transcription Factors / metabolism* YAP-Signaling Proteins
IF 12.121
Times Cited 28
WOS Category CELL BIOLOGY
Resource
Drosophila 12072R-1