| RRC ID |
35965
|
| Author |
Kurokawa Y, Noda T, Yamagata Y, Angeles-Shim R, Sunohara H, Uehara K, Furuta T, Nagai K, Jena KK, Yasui H, Yoshimura A, Ashikari M, Doi K.
|
| Title |
Construction of a versatile SNP array for pyramiding useful genes of rice.
|
| Journal |
Plant Sci
|
| Abstract |
DNA marker-assisted selection (MAS) has become an indispensable component of breeding. Single nucleotide polymorphisms (SNP) are the most frequent polymorphism in the rice genome. However, SNP markers are not readily employed in MAS because of limitations in genotyping platforms. Here the authors report a Golden Gate SNP array that targets specific genes controlling yield-related traits and biotic stress resistance in rice. As a first step, the SNP genotypes were surveyed in 31 parental varieties using the Affymetrix Rice 44K SNP microarray. The haplotype information for 16 target genes was then converted to the Golden Gate platform with 143-plex markers. Haplotypes for the 14 useful allele are unique and can discriminate among all other varieties. The genotyping consistency between the Affymetrix microarray and the Golden Gate array was 92.8%, and the accuracy of the Golden Gate array was confirmed in 3 F2 segregating populations. The concept of the haplotype-based selection by using the constructed SNP array was proofed.
|
| Volume |
242
|
| Pages |
131-139
|
| Published |
2016-1-1
|
| DOI |
10.1016/j.plantsci.2015.09.008
|
| PII |
S0168-9452(15)30065-0
|
| PMID |
26566831
|
| MeSH |
Base Sequence
Gene Frequency
Genes, Plant / genetics*
Genetics, Population / methods
Genome, Plant / genetics
Genotype
Genotyping Techniques / methods
Haplotypes*
Microarray Analysis / methods
Molecular Sequence Data
Oryza / classification
Oryza / genetics*
Plant Breeding / methods
Polymorphism, Single Nucleotide*
Reproducibility of Results
Selective Breeding
Sequence Homology, Nucleic Acid
|
| IF |
3.591
|
| Times Cited |
13
|
|
WOS Category
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Rice |
T65 |
