Reference - Detail
| RRC ID | 35966 |
|---|---|
| Author | Tomioka M, Nishimoto M, Miyagi S, Katayanagi T, Fukui N, Niwa H, Muramatsu M, Okuda A. |
| Title | Identification of Sox-2 regulatory region which is under the control of Oct-3/4-Sox-2 complex. |
| Journal | Nucleic Acids Res |
| Abstract |
Sox-2 is a transcriptional cofactor expressed in embryonic stem (ES) cells as well as in neuronal cells. It has been demonstrated that Sox-2 plays an important role in supporting gene expression in ES cells, especially by forming a complex with embryonic Octamer factor, Oct-3/4. Here, we have analyzed the regulatory regions of the Sox-2 gene and identified two enhancers which stimulate transcription in ES cells as well as in embryonal carcinoma cells. These regulatory regions, which we termed Sox regulatory regions (SRR) 1 and 2, exert their function specifically when cells are in an undifferentiated state. Interestingly, like the regulatory elements of FGF-4 and UTF1 genes, combinatorial action of Octamer and Sox-2 binding sites support the SRR2 activity. However, biochemical analyses reveal that, due to the unique sequence and/or its organization, the SRR2 bears distinct characteristics from those of FGF-4 and UTF1 regulatory elements. That is, unlike the FGF-4 gene enhancer, the SRR2 precludes the binding of the Oct-1-Sox-2 complex. The difference between the SRR2 and UTF1 regulatory element is in the ability of SRR2 to recruit the Oct-6-Sox-2 complex as well as the Oct-3/4-Sox-2 complex. Co-transfection analyses confirm that both complexes are able to stimulate transcription through the SRR2 element. |
| Volume | 30(14) |
| Pages | 3202-13 |
| Published | 2002-7-15 |
| DOI | 10.1093/nar/gkf435 |
| PMID | 12136102 |
| PMC | PMC135755 |
| MeSH | Animals Base Sequence Binding Sites / genetics COS Cells Cell Line Chromosomal Proteins, Non-Histone Cloning, Molecular DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* HMGB Proteins Luciferases / genetics Luciferases / metabolism Macromolecular Substances Mice Mice, Inbred Strains Molecular Sequence Data Nuclear Proteins / genetics Nuclear Proteins / metabolism* Octamer Transcription Factor-3 Octamer Transcription Factor-6 Plasmids / genetics Protein Binding Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Regulatory Sequences, Nucleic Acid / genetics* SOXB1 Transcription Factors Trans-Activators / genetics Trans-Activators / metabolism Transcription Factors / genetics Transcription Factors / metabolism* Transcription, Genetic Transfection Tumor Cells, Cultured |
| IF | 11.502 |
| Times Cited | 195 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| DNA material | pSRR2 tk-luc (RDB07095) |