RRC ID 35968
Author Sasamoto Y, Hayashi R, Park SJ, Saito-Adachi M, Suzuki Y, Kawasaki S, Quantock AJ, Nakai K, Tsujikawa M, Nishida K.
Title PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes.
Journal Sci Rep
Abstract PAX6 is the key transcription factor involved in eye development in humans, but the differential functions of the two PAX6 isoforms, isoform-a and isoform-b, are largely unknown. To reveal their function in the corneal epithelium, PAX6 isoforms, along with reprogramming factors, were transduced into human non-ocular epithelial cells. Herein, we show that the two PAX6 isoforms differentially and cooperatively regulate the expression of genes specific to the structure and functions of the corneal epithelium, particularly keratin 3 (KRT3) and keratin 12 (KRT12). PAX6 isoform-a induced KRT3 expression by targeting its upstream region. KLF4 enhanced this induction. A combination of PAX6 isoform-b, KLF4, and OCT4 induced KRT12 expression. These new findings will contribute to furthering the understanding of the molecular basis of the corneal epithelium specific phenotype.
Volume 6
Pages 20807
Published 2016-2-22
DOI 10.1038/srep20807
PII srep20807
PMID 26899008
PMC PMC4761963
MeSH Cell Line Epithelial Cells / metabolism Epithelium, Corneal / growth & development Epithelium, Corneal / metabolism Eye / growth & development* Eye / metabolism Gene Expression Regulation, Developmental Humans Keratin-12 / biosynthesis* Keratin-12 / genetics Keratin-3 / biosynthesis* Keratin-3 / genetics Kruppel-Like Factor 4 Kruppel-Like Transcription Factors / biosynthesis* Kruppel-Like Transcription Factors / genetics Octamer Transcription Factor-3 / biosynthesis* Octamer Transcription Factor-3 / genetics PAX6 Transcription Factor / biosynthesis PAX6 Transcription Factor / genetics* Protein Isoforms / biosynthesis Protein Isoforms / genetics Transduction, Genetic
IF 3.998
Times Cited 20
DNA material CSII-CMV-MCS-IRES2-Venus (RDB04383) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393).