| RRC ID |
36044
|
| 著者 |
Shibata M, Banno R, Sugiyama M, Tominaga T, Onoue T, Tsunekawa T, Azuma Y, Hagiwara D, Lu W, Ito Y, Goto M, Suga H, Sugimura Y, Oiso Y, Arima H.
|
| タイトル |
AgRP Neuron-Specific Deletion of Glucocorticoid Receptor Leads to Increased Energy Expenditure and Decreased Body Weight in Female Mice on a High-Fat Diet.
|
| ジャーナル |
Endocrinology
|
| Abstract |
Agouti-related protein (AgRP) expressed in the arcuate nucleus is a potent orexigenic neuropeptide, which increases food intake and reduces energy expenditure resulting in increases in body weight (BW). Glucocorticoids, key hormones that regulate energy balance, have been shown in rodents to regulate the expression of AgRP. In this study, we generated AgRP-specific glucocorticoid receptor (GR)-deficient (knockout [KO]) mice. Female and male KO mice on a high-fat diet (HFD) showed decreases in BW at the age of 6 weeks compared with wild-type mice, and the differences remained significant until 16 weeks old. The degree of resistance to diet-induced obesity was more robust in female than in male mice. On a chow diet, the female KO mice showed slightly but significantly attenuated weight gain compared with wild-type mice after 11 weeks, whereas there were no significant differences in BW in males between genotypes. Visceral fat pad mass was significantly decreased in female KO mice on HFD, whereas there were no significant differences in lean body mass between genotypes. Although food intake was similar between genotypes, oxygen consumption was significantly increased in female KO mice on HFD. In addition, the uncoupling protein-1 expression in the brown adipose tissues was increased in KO mice. These data demonstrate that the absence of GR signaling in AgRP neurons resulted in increases in energy expenditure accompanied by decreases in adiposity in mice fed HFD, indicating that GR signaling in AgRP neurons suppresses energy expenditure under HFD conditions.
|
| 巻・号 |
157(4)
|
| ページ |
1457-66
|
| 公開日 |
2016-4-1
|
| DOI |
10.1210/en.2015-1430
|
| PMID |
26889940
|
| MeSH |
Agouti-Related Protein / genetics
Agouti-Related Protein / metabolism*
Animals
Arcuate Nucleus of Hypothalamus / cytology
Arcuate Nucleus of Hypothalamus / metabolism
Blotting, Western
Body Weight / genetics
Body Weight / physiology*
Diet, High-Fat / adverse effects
Eating / genetics
Eating / physiology
Energy Metabolism / genetics
Energy Metabolism / physiology*
Female
Gene Expression
In Situ Hybridization
Intra-Abdominal Fat / metabolism
Male
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Microscopy, Confocal
Neurons / metabolism
Obesity / etiology
Obesity / genetics
Obesity / metabolism
Receptors, Glucocorticoid / genetics
Receptors, Glucocorticoid / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
|
| IF |
3.934
|
| 引用数 |
12
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
|
| リソース情報 |
| 実験動物マウス |
RBRC04874 |
