RRC ID 36129
著者 Mevius DE, Shen Y, Morishita M, di Luccio E.
タイトル Cloning, expression, purification and crystallization of Schizosaccharomyces pombe Set7, a putative histone methyltransferase.
ジャーナル Acta Crystallogr F Struct Biol Commun
Abstract Dysfunction of histone-modifying enzymes affects chromatin regulation and is involved in carcinogenesis, tumour progression and other diseases. Histone methyltransferases are a family of key histone-modifying enzymes, but their structures, functions and mechanisms are incompletely understood, thus constraining drug-design efforts. Here, preliminary steps towards structure-function studies of Schizosaccharomyces pombe Set7, a putative histone methyltransferase and the first yeast full-length SET-domain-containing protein to be studied using X-ray crystallography, are reported. The methods from cloning to X-ray diffraction and phasing are discussed and the results will aid in prospective studies of histone-modifying enzymes.
巻・号 72(Pt 4)
ページ 263-8
公開日 2016-4-1
DOI 10.1107/S2053230X16003794
PII S2053230X16003794
PMID 27050258
PMC PMC4822981
MeSH Amino Acid Sequence Base Sequence Cloning, Molecular Crystallization Histone Methyltransferases Histone-Lysine N-Methyltransferase Schizosaccharomyces / chemistry* Schizosaccharomyces pombe Proteins / chemistry Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / isolation & purification*
IF 0.968
引用数 1
WOS 分野 BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY BIOPHYSICS CRYSTALLOGRAPHY
リソース情報
酵母 cDNA library