RRC ID 36131
Author Hisano H, Matsuura T, Mori IC, Yamane M, Sato K.
Title Endogenous hormone levels affect the regeneration ability of callus derived from different organs in barley.
Journal Plant Physiol Biochem
Abstract Hordeum vulgare (barley) is an important agricultural crop worldwide. A simple and efficient transformation system is needed to analyze the functions of barley genes and generate lines with improved agronomic traits. Currently, Golden Promise and Igri are the most amenable barley cultivars for stable transformation. Here we evaluated the regeneration ratios and endogenous hormone levels of calli derived from various malting barley cultivars, including Golden Promise, Haruna Nijo, and Morex. We harvested samples not only from immature embryos, but also from different explants of juvenile plants, cotyledons, coleoptiles, and roots. The callus properties differed among genotypes and explant types. Calli derived from the immature embryos of Golden Promise, which showed the highest ratio of regeneration of green shoots, had the highest contents of indoleacetic acid, trans-zeatin, and cis-zeatin. By contrast, calli derived from the cotyledons of Morex and the immature embryos of Haruna Nijo had elevated levels of salicylic acid and abscisic acid, respectively. We thus propose that the former phytohormones are positively associated with the regeneration ability of callus but the later phytohormones are negatively associated.
Volume 99
Pages 66-72
Published 2016-2-1
DOI 10.1016/j.plaphy.2015.12.005
PII S0981-9428(15)30183-2
PMID 26735586
MeSH Hordeum / physiology* Plant Growth Regulators / metabolism* Seeds / physiology*
IF 3.72
Times Cited 13
Barley Barley seed samples