RRC ID 36139
著者 Enya S, Daimon T, Igarashi F, Kataoka H, Uchibori M, Sezutsu H, Shinoda T, Niwa R.
タイトル The silkworm glutathione S-transferase gene noppera-bo is required for ecdysteroid biosynthesis and larval development.
ジャーナル Insect Biochem Mol Biol
Abstract Insect molting and metamorphosis are tightly controlled by ecdysteroids, which are important steroid hormones that are synthesized from dietary sterols in the prothoracic gland. One of the ecdysteroidogenic genes in the fruit fly Drosophila melanogaster is noppera-bo (nobo), also known as GSTe14, which encodes a member of the epsilon class of glutathione S-transferases. In D. melanogaster, nobo plays a crucial role in utilizing cholesterol via regulating its transport and/or metabolism in the prothoracic gland. However, it is still not known whether the orthologs of nobo from other insects are also involved in ecdysteroid biosynthesis via cholesterol transport and/or metabolism in the prothoracic gland. Here we report genetic evidence showing that the silkworm Bombyx mori ortholog of nobo (nobo-Bm; GSTe7) is essential for silkworm development. nobo-Bm is predominantly expressed in the prothoracic gland. To assess the functional importance of nobo-Bm, we generated a B. mori genetic mutant of nobo-Bm using TALEN-mediated genome editing. We show that loss of nobo-Bm function causes larval arrest and a glossy cuticle phenotype, which are rescued by the application of 20-hydroxyecdysone. Moreover, the prothoracic gland cells isolated from the nobo-Bm mutant exhibit an abnormal accumulation of 7-dehydrocholesterol, a cholesterol metabolite. These results suggest that the nobo family of glutathione S-transferases is essential for development and for the regulation of sterol utilization in the prothoracic gland in not only the Diptera but also the Lepidoptera. On the other hand, loss of nobo function mutants of D. melanogaster and B. mori abnormally accumulates different sterols, implying that the sterol utilization in the PG is somewhat different between these two insect species.
巻・号 61
ページ 1-7
公開日 2015-6-1
DOI 10.1016/j.ibmb.2015.04.001
PII S0965-1748(15)00068-5
PMID 25881968
MeSH Animals Bombyx / genetics Bombyx / growth & development* Bombyx / metabolism* Dehydrocholesterols / metabolism Ecdysteroids / biosynthesis* Ecdysterone / metabolism Genes, Insect Glutathione Transferase / genetics Glutathione Transferase / metabolism* Insect Proteins / genetics Insect Proteins / metabolism* Larva / genetics Larva / growth & development Larva / metabolism
IF 3.827
引用数 26
WOS 分野 ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
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