| RRC ID |
36145
|
| 著者 |
Fukui T, Kawamoto M, Shoji K, Kiuchi T, Sugano S, Shimada T, Suzuki Y, Katsuma S.
|
| タイトル |
The Endosymbiotic Bacterium Wolbachia Selectively Kills Male Hosts by Targeting the Masculinizing Gene.
|
| ジャーナル |
PLoS Pathog
|
| Abstract |
Pathogens are known to manipulate the reproduction and development of their hosts for their own benefit. Wolbachia is an endosymbiotic bacterium that infects a wide range of insect species. Wolbachia is known as an example of a parasite that manipulates the sex of its host's progeny. Infection of Ostrinia moths by Wolbachia causes the production of all-female progeny, however, the mechanism of how Wolbachia accomplishes this male-specific killing is unknown. Here we show for the first time that Wolbachia targets the host masculinizing gene of Ostrinia to accomplish male-killing. We found that Wolbachia-infected O. furnacalis embryos do not express the male-specific splice variant of doublesex, a gene which acts at the downstream end of the sex differentiation cascade, throughout embryonic development. Transcriptome analysis revealed that Wolbachia infection markedly reduces the mRNA level of Masc, a gene that encodes a protein required for both masculinization and dosage compensation in the silkworm Bombyx mori. Detailed bioinformatic analysis also elucidated that dosage compensation of Z-linked genes fails in Wolbachia-infected O. furnacalis embryos, a phenomenon that is extremely similar to that observed in Masc mRNA-depleted male embryos of B. mori. Finally, injection of in vitro transcribed Masc cRNA into Wolbachia-infected embryos rescued male progeny. Our results show that Wolbachia-induced male-killing is caused by a failure of dosage compensation via repression of the host masculinizing gene. Our study also shows a novel strategy by which a pathogen hijacks the host sex determination cascade.
|
| 巻・号 |
11(7)
|
| ページ |
e1005048
|
| 公開日 |
2015-7-1
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| DOI |
10.1371/journal.ppat.1005048
|
| PII |
PPATHOGENS-D-15-01368
|
| PMID |
26172536
|
| PMC |
PMC4501725
|
| MeSH |
Animals
Female
Male
Molecular Sequence Data
Moths / genetics
Moths / parasitology*
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction
Transfection
Wolbachia*
Zinc Fingers / genetics
|
| IF |
6.218
|
| 引用数 |
21
|
|
WOS 分野
|
PARASITOLOGY
MICROBIOLOGY
VIROLOGY
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| リソース情報 |
| カイコ |
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