論文 - 詳細
| RRC ID | 36145 |
|---|---|
| 著者 | Fukui T, Kawamoto M, Shoji K, Kiuchi T, Sugano S, Shimada T, Suzuki Y, Katsuma S. |
| タイトル | The Endosymbiotic Bacterium Wolbachia Selectively Kills Male Hosts by Targeting the Masculinizing Gene. |
| ジャーナル | PLoS Pathog |
| Abstract |
Pathogens are known to manipulate the reproduction and development of their hosts for their own benefit. Wolbachia is an endosymbiotic bacterium that infects a wide range of insect species. Wolbachia is known as an example of a parasite that manipulates the sex of its host's progeny. Infection of Ostrinia moths by Wolbachia causes the production of all-female progeny, however, the mechanism of how Wolbachia accomplishes this male-specific killing is unknown. Here we show for the first time that Wolbachia targets the host masculinizing gene of Ostrinia to accomplish male-killing. We found that Wolbachia-infected O. furnacalis embryos do not express the male-specific splice variant of doublesex, a gene which acts at the downstream end of the sex differentiation cascade, throughout embryonic development. Transcriptome analysis revealed that Wolbachia infection markedly reduces the mRNA level of Masc, a gene that encodes a protein required for both masculinization and dosage compensation in the silkworm Bombyx mori. Detailed bioinformatic analysis also elucidated that dosage compensation of Z-linked genes fails in Wolbachia-infected O. furnacalis embryos, a phenomenon that is extremely similar to that observed in Masc mRNA-depleted male embryos of B. mori. Finally, injection of in vitro transcribed Masc cRNA into Wolbachia-infected embryos rescued male progeny. Our results show that Wolbachia-induced male-killing is caused by a failure of dosage compensation via repression of the host masculinizing gene. Our study also shows a novel strategy by which a pathogen hijacks the host sex determination cascade. |
| 巻・号 | 11(7) |
| ページ | e1005048 |
| 公開日 | 2015-7-1 |
| DOI | 10.1371/journal.ppat.1005048 |
| PII | PPATHOGENS-D-15-01368 |
| PMID | 26172536 |
| PMC | PMC4501725 |
| MeSH | Animals Female Male Molecular Sequence Data Moths / genetics Moths / parasitology* Phylogeny Reverse Transcriptase Polymerase Chain Reaction Transfection Wolbachia* Zinc Fingers / genetics |
| IF | 6.218 |
| 引用数 | 21 |
| WOS 分野 | PARASITOLOGY MICROBIOLOGY VIROLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 20 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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