論文 - 詳細
| RRC ID | 36152 |
|---|---|
| 著者 | Gao J, Wang J, Wang W, Liu C, Meng Y. |
| タイトル | Isolation, purification, and identification of an important pigment, sepiapterin, from integument of the lemon mutant of the silkworm, Bombyx mori. |
| ジャーナル | J Insect Sci |
| Abstract |
Sepiapterin is the precursor of tetrahydrobiopterin, an important coenzyme of aromatic amino acid hydroxylases, the lack of which leads to a variety of physiological metabolic diseases or neurological syndromes in humans. Sepiapterin is a main pigment component in the integument of the lemon mutant of the silkworm, Bombyx mori (L.) (Lepidoptera: Bombycidae), and is present there in extremely high content, so lemon is a valuable genetic resource to extract sepiapterin. In this study, an effective experimental system was set up for isolation and purification of sepiapterin from lemon silkworms by optimizing homogenization solvent, elution buffer, and separation chromatographic column. The results showed that ethanol was the most suitable solvent to homogenize the integument, with a concentration of 50% and solid:liquid ratio of 1:20 (g/mL). Sepiapterin was purified successively by column chromatography of cellulose Ecteola, sephadex G-25-150, and cellulose phosphate, and was identified by ultraviolet-visible absorption spectrometry. A stable and accurate high performance liquid chromatography method was constructed to identify sepiapterin and conduct qualitative and quantitative analyses. Sepiapterin of high purity was achieved, and the harvest reached about 40 ug/g of integument in the experiments. This work helps to obtaining natural sepiapterin in large amounts in order to use the lemon B. mori mutant to produce BH4 in vitro. |
| 巻・号 | 13 |
| ページ | 118 |
| 公開日 | 2013-1-1 |
| DOI | 10.1673/031.013.11801 |
| PMID | 24773269 |
| PMC | PMC4011334 |
| MeSH | Animals Bombyx / chemistry* Bombyx / genetics Bombyx / growth & development Chromatography, High Pressure Liquid* Integumentary System / physiology Larva / chemistry Larva / genetics Pterins / chemistry Pterins / isolation & purification* |
| IF | 1.325 |
| 引用数 | 2 |
| WOS 分野 | ENTOMOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カイコ | p50 l70 |