RRC ID 36182
Author Pankin A, Campoli C, Dong X, Kilian B, Sharma R, Himmelbach A, Saini R, Davis SJ, Stein N, Schneeberger K, von Korff M.
Title Mapping-by-sequencing identifies HvPHYTOCHROME C as a candidate gene for the early maturity 5 locus modulating the circadian clock and photoperiodic flowering in barley.
Journal Genetics
Abstract Phytochromes play an important role in light signaling and photoperiodic control of flowering time in plants. Here we propose that the red/far-red light photoreceptor HvPHYTOCHROME C (HvPHYC), carrying a mutation in a conserved region of the GAF domain, is a candidate underlying the early maturity 5 locus in barley (Hordeum vulgare L.). We fine mapped the gene using a mapping-by-sequencing approach applied on the whole-exome capture data from bulked early flowering segregants derived from a backcross of the Bowman(eam5) introgression line. We demonstrate that eam5 disrupts circadian expression of clock genes. Moreover, it interacts with the major photoperiod response gene Ppd-H1 to accelerate flowering under noninductive short days. Our results suggest that HvPHYC participates in transmission of light signals to the circadian clock and thus modulates light-dependent processes such as photoperiodic regulation of flowering.
Volume 198(1)
Pages 383-96
Published 2014-9-1
DOI 10.1534/genetics.114.165613
PII genetics.114.165613
PMID 24996910
PMC PMC4174949
MeSH Circadian Clocks / genetics* Exome Flowers / genetics* Flowers / growth & development Genes, Plant Hordeum / genetics* Hordeum / growth & development Hordeum / physiology Inbreeding Photoperiod Physical Chromosome Mapping Phytochrome / genetics* Plant Development / genetics Plant Proteins / genetics* Quantitative Trait Loci*
IF 4.015
Times Cited 43
Barley Barley seed samples