RRC ID 36193
Author Oda K, Otani M, Uraguchi S, Akihiro T, Fujiwara T.
Title Rice ABCG43 is Cd inducible and confers Cd tolerance on yeast.
Journal Biosci Biotechnol Biochem
Abstract An understanding of the cadmium (Cd) transport system in rice can serve as a basis for coping with Cd-related problems in rice and human health. To identify a new gene involved in Cd transport in rice, we screened our yeast library, expressing 140 kinds of rice ATP binding cassette (ABC)-type transporters. From the screening, we found that OsABCG43/PDR5 conferred high Cd tolerance on yeast. The Cd concentration of yeast carrying OsABCG43 was similar to that of the vector control. The OsABCG43 transcript was detected both in shoots and roots. Accumulation of it was elevated by Cd treatment in the roots but not in the shoots. This study indicates that OsABCG43 is a Cd inducible-transporter gene capable of conferring Cd tolerance on yeast.
Volume 75(6)
Pages 1211-3
Published 2011-1-1
DOI 10.1271/bbb.110193
PII JST.JSTAGE/bbb/110193
PMID 21670506
MeSH ATP-Binding Cassette Transporters / genetics ATP-Binding Cassette Transporters / metabolism* Cadmium / metabolism Cadmium / pharmacology* Cloning, Molecular Gene Expression Regulation, Plant Humans Oryza / drug effects Oryza / genetics Oryza / metabolism* Plant Proteins / genetics Plant Proteins / metabolism* Plant Roots / drug effects Plant Roots / genetics Plant Roots / metabolism* Plant Shoots / drug effects Plant Shoots / genetics Plant Shoots / metabolism* Plasmids Saccharomyces cerevisiae / drug effects Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism* Transformation, Genetic
IF 1.516
Times Cited 25
WOS Category FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Yeast w303-1a