Reference - Detail
| RRC ID | 3621 |
|---|---|
| Author | Matsushita-Ishiodori Y, Yamanaka K, Ogura T. |
| Title | The C. elegans homologue of the spastic paraplegia protein, spastin, disassembles microtubules. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Mutations in human spastin (SPG4) cause an autosomal dominant form of hereditary spastic paraplegia. Sequence analysis revealed that spastin contains the AAA (ATPases associated with diverse cellular activities) domain in the C-terminal region. Recently, it was reported that spastin interacts dynamically with microtubules and displays microtubule-severing activity. A plausible Caenorhabditis elegans homologue of spastin (SPAS-1) has been identified by homology search and phylogenetic analyses. To understand the function of the spastin homologue, we characterized the spas-1 deletion mutant and analyzed spas-1 expression regulation in C. elegans. SPAS-1 was localized with cytoskeletons at the perinuclear region. We found that microtubules were intensely stained at the centrosomal region in the deletion mutant. Furthermore, overexpression of SPAS-1 caused disassembly of microtubule network in cultured cells, while ATPase-deficient SPAS-1 did not. These results indicate that C. elegans SPAS-1 plays an important role in microtubule dynamics. We also found that two kinds of products were generated from spas-1 by alternative splicing in a developmental stage-dependent manner. |
| Volume | 359(1) |
| Pages | 157-62 |
| Published | 2007-7-20 |
| DOI | 10.1016/j.bbrc.2007.05.086 |
| PII | S0006-291X(07)01050-9 |
| PMID | 17531954 |
| MeSH | Adenosine Triphosphatases / chemistry* Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism* Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Microtubules / metabolism* Microtubules / ultrastructure* Organ Specificity Spastin Tissue Distribution |
| IF | 2.985 |
| Times Cited | 20 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| C.elegans | tm683 |