RRC ID 36222
著者 Nishino K, Obara K, Kihara A.
タイトル The C-terminal Cytosolic Region of Rim21 Senses Alterations in Plasma Membrane Lipid Composition: INSIGHTS INTO SENSING MECHANISMS FOR PLASMA MEMBRANE LIPID ASYMMETRY.
ジャーナル J Biol Chem
Abstract Yeast responds to alterations in plasma membrane lipid asymmetry and external alkalization via the sensor protein Rim21 in the Rim101 pathway. However, the sensing mechanism used by Rim21 remains unclear. Here, we found that the C-terminal cytosolic domain of Rim21 (Rim21C) fused with GFP was associated with the plasma membrane under normal conditions but dissociated upon alterations in lipid asymmetry or external alkalization. This indicates that Rim21C contains a sensor motif. Rim21C contains multiple clusters of charged residues. Among them, three consecutive Glu residues (EEE motif) were essential for Rim21 function and dissociation of Rim21C from the plasma membrane in response to changes in lipid asymmetry. In contrast, positively charged residues adjacent to the EEE motif were required for Rim21C to associate with the membrane. We therefore propose an "antenna hypothesis," in which Rim21C moves to or from the plasma membrane and functions as the sensing mechanism of Rim21.
巻・号 290(52)
ページ 30797-805
公開日 2015-12-25
DOI 10.1074/jbc.M115.674382
PII S0021-9258(20)39282-6
PMID 26527678
PMC PMC4692209
MeSH Amino Acid Motifs Cell Membrane / chemistry Cell Membrane / genetics Cell Membrane / metabolism* Lipid Metabolism Lipids / chemistry* Protein Structure, Tertiary Receptors, Cell Surface / chemistry* Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism* Saccharomyces cerevisiae / chemistry Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / chemistry* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Signal Transduction
IF 4.238
引用数 12
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
酵母 BYP6740