RRC ID |
36222
|
Author |
Nishino K, Obara K, Kihara A.
|
Title |
The C-terminal Cytosolic Region of Rim21 Senses Alterations in Plasma Membrane Lipid Composition: INSIGHTS INTO SENSING MECHANISMS FOR PLASMA MEMBRANE LIPID ASYMMETRY.
|
Journal |
J Biol Chem
|
Abstract |
Yeast responds to alterations in plasma membrane lipid asymmetry and external alkalization via the sensor protein Rim21 in the Rim101 pathway. However, the sensing mechanism used by Rim21 remains unclear. Here, we found that the C-terminal cytosolic domain of Rim21 (Rim21C) fused with GFP was associated with the plasma membrane under normal conditions but dissociated upon alterations in lipid asymmetry or external alkalization. This indicates that Rim21C contains a sensor motif. Rim21C contains multiple clusters of charged residues. Among them, three consecutive Glu residues (EEE motif) were essential for Rim21 function and dissociation of Rim21C from the plasma membrane in response to changes in lipid asymmetry. In contrast, positively charged residues adjacent to the EEE motif were required for Rim21C to associate with the membrane. We therefore propose an "antenna hypothesis," in which Rim21C moves to or from the plasma membrane and functions as the sensing mechanism of Rim21.
|
Volume |
290(52)
|
Pages |
30797-805
|
Published |
2015-12-25
|
DOI |
10.1074/jbc.M115.674382
|
PII |
S0021-9258(20)39282-6
|
PMID |
26527678
|
PMC |
PMC4692209
|
MeSH |
Amino Acid Motifs
Cell Membrane / chemistry
Cell Membrane / genetics
Cell Membrane / metabolism*
Lipid Metabolism
Lipids / chemistry*
Protein Structure, Tertiary
Receptors, Cell Surface / chemistry*
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism*
Saccharomyces cerevisiae / chemistry
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / chemistry*
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / metabolism*
Signal Transduction
|
IF |
4.238
|
Times Cited |
12
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
Resource |
Yeast |
BYP6740 |