RRC ID 36222
Author Nishino K, Obara K, Kihara A.
Title The C-terminal Cytosolic Region of Rim21 Senses Alterations in Plasma Membrane Lipid Composition: INSIGHTS INTO SENSING MECHANISMS FOR PLASMA MEMBRANE LIPID ASYMMETRY.
Journal J. Biol. Chem.
Abstract Yeast responds to alterations in plasma membrane lipid asymmetry and external alkalization via the sensor protein Rim21 in the Rim101 pathway. However, the sensing mechanism used by Rim21 remains unclear. Here, we found that the C-terminal cytosolic domain of Rim21 (Rim21C) fused with GFP was associated with the plasma membrane under normal conditions but dissociated upon alterations in lipid asymmetry or external alkalization. This indicates that Rim21C contains a sensor motif. Rim21C contains multiple clusters of charged residues. Among them, three consecutive Glu residues (EEE motif) were essential for Rim21 function and dissociation of Rim21C from the plasma membrane in response to changes in lipid asymmetry. In contrast, positively charged residues adjacent to the EEE motif were required for Rim21C to associate with the membrane. We therefore propose an "antenna hypothesis," in which Rim21C moves to or from the plasma membrane and functions as the sensing mechanism of Rim21.
Volume 290(52)
Pages 30797-805
Published 2015-12-25
DOI 10.1074/jbc.M115.674382
PII M115.674382
PMID 26527678
PMC PMC4692209
MeSH Amino Acid Motifs Cell Membrane / chemistry Cell Membrane / genetics Cell Membrane / metabolism* Lipid Metabolism Lipids / chemistry* Protein Structure, Tertiary Receptors, Cell Surface / chemistry* Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism* Saccharomyces cerevisiae / chemistry Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / chemistry* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Signal Transduction
IF 4.011
Times Cited 3
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Yeast BYP6740