RRC ID 3623
著者 O'Rourke SM, Dorfman MD, Carter JC, Bowerman B.
タイトル Dynein modifiers in C. elegans: light chains suppress conditional heavy chain mutants.
ジャーナル PLoS Genet
Abstract Cytoplasmic dynein is a microtubule-dependent motor protein that functions in mitotic cells during centrosome separation, metaphase chromosome congression, anaphase spindle elongation, and chromosome segregation. Dynein is also utilized during interphase for vesicle transport and organelle positioning. While numerous cellular processes require cytoplasmic dynein, the mechanisms that target and regulate this microtubule motor remain largely unknown. By screening a conditional Caenorhabditis elegans cytoplasmic dynein heavy chain mutant at a semipermissive temperature with a genome-wide RNA interference library to reduce gene functions, we have isolated and characterized twenty dynein-specific suppressor genes. When reduced in function, these genes suppress dynein mutants but not other conditionally mutant loci, and twelve of the 20 specific suppressors do not exhibit sterile or lethal phenotypes when their function is reduced in wild-type worms. Many of the suppressor proteins, including two dynein light chains, localize to subcellular sites that overlap with those reported by others for the dynein heavy chain. Furthermore, knocking down any one of four putative dynein accessory chains suppresses the conditional heavy chain mutants, suggesting that some accessory chains negatively regulate heavy chain function. We also identified 29 additional genes that, when reduced in function, suppress conditional mutations not only in dynein but also in loci required for unrelated essential processes. In conclusion, we have identified twenty genes that in many cases are not essential themselves but are conserved and when reduced in function can suppress conditionally lethal C. elegans cytoplasmic dynein heavy chain mutants. We conclude that conserved but nonessential genes contribute to dynein function during the essential process of mitosis.
巻・号 3(8)
ページ e128
公開日 2007-8-1
DOI 10.1371/journal.pgen.0030128
PII 07-PLGE-RA-0201
PMID 17676955
PMC PMC1937013
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / genetics* Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / antagonists & inhibitors* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / physiology Carrier Proteins / genetics Carrier Proteins / physiology* Cytoplasm / enzymology* Cytoplasm / genetics* Cytoplasm / physiology Cytoplasmic Dyneins Drosophila Proteins / genetics Drosophila Proteins / physiology* Dyneins / antagonists & inhibitors Dyneins / genetics Dyneins / physiology* Protein Subunits / genetics Protein Subunits / physiology Repressor Proteins / genetics Repressor Proteins / physiology*
IF 5.175
引用数 54
WOS 分野 GENETICS & HEREDITY
リソース情報
線虫 tm1380 tm2645