RRC ID 36281
著者 Zhu L, Tatsuke T, Xu J, Li Z, Mon H, Lee JM, Kusakabe T.
タイトル Loqs depends on R2D2 to localize in D2 body-like granules and functions in RNAi pathways in silkworm cells.
ジャーナル Insect Biochem Mol Biol
Abstract The phenomenon of RNA interference (RNAi) has been found in various organisms. However, the proteins implicated in RNAi pathway in different species show distinct roles. Knowledge on the underlying mechanism of lepidopteron RNAi is quite lacking such as the roles of Loquacious (Loqs) and R2D2, the dsRNA-binding proteins in silkworm RNAi pathway. Here, we report that Loqs and R2D2 protein depletion affected efficiency of dsRNA-mediated RNAi pathway. Besides, Loqs was found to co-localize with Dicer2 to some specific cytoplasmic foci, which were looked like D2-bodies marked by R2D2 and Dicer2 in Fly cells, thereby calling the foci as D2 body-like granules. Using RNAi methods, Loqs was found to be the key protein in these granules, although R2D2 determined the localization of Loqs in D2 body-like granules. Interestingly, in the R2D2-depeted silkworm cells, the formation of processing bodies, another cytoplasmic foci, was affected. These data indicated R2D2 regulated these two kinds of cytoplasmic foci. Domain deletion analysis demonstrated that dsRBD 1 and 2 were required for Loqs in D2 body-like granules and dsRBD 2 and 3 were required for Loqs to interact with R2D2 and Ago1, respectively. Altogether, our observations provide important information for further study on D2 body-like granules, the newly found cytoplasmic foci in silkworm cells.
巻・号 64
ページ 78-90
公開日 2015-9-1
DOI 10.1016/j.ibmb.2015.07.011
PII S0965-1748(15)30028-X
PMID 26184783
MeSH Animals Bombyx / genetics* Cell Line Cytoplasmic Granules / metabolism* Gene Deletion Insect Proteins / genetics Insect Proteins / metabolism* RNA Interference* RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism*
IF 3.827
引用数 2
WOS 分野 ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
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