Reference - Detail
| RRC ID | 36329 |
|---|---|
| Author | Gupta AK, Mita K, Arunkumar KP, Nagaraju J. |
| Title | Molecular architecture of silk fibroin of Indian golden silkmoth, Antheraea assama. |
| Journal | Sci Rep |
| Abstract |
The golden silk spun by Indian golden silkmoth Antheraea assama, is regarded for its shimmering golden luster, tenacity and value as biomaterial. This report describes the gene coding for golden silk H-fibroin (AaFhc), its expression, full-length sequence and structurally important motifs discerning the underlying genetic and biochemical factors responsible for its much sought-after properties. The coding region, with biased isocodons, encodes highly repetitious crystalline core, flanked by a pair of 5' and 3' non-repetitious ends. AaFhc mRNA expression is strictly territorial, confined to the posterior silk gland, encoding a protein of size 230 kDa, which makes homodimers making the elementary structural units of the fibrous core of the golden silk. Characteristic polyalanine repeats that make tight β-sheet crystals alternate with non-polyalanine repeats that make less orderly antiparallel β-sheets, β-turns and partial α-helices. Phylogenetic analysis of the conserved N-terminal amorphous motif and the comparative analysis of the crystalline region with other saturniid H-fibroins reveal that AaFhc has longer, numerous and relatively uniform repeat motifs with lower serine content that assume tighter β-crystals and denser packing, which are speculated to be responsible for its acclaimed properties of higher tensile strength and higher refractive index responsible for golden luster. |
| Volume | 5 |
| Pages | 12706 |
| Published | 2015-8-3 |
| DOI | 10.1038/srep12706 |
| PII | srep12706 |
| PMID | 26235912 |
| PMC | PMC4522600 |
| MeSH | Amino Acid Motifs Animals Base Sequence Fibroins / chemistry Fibroins / genetics* Fibroins / metabolism Genes Insect Proteins / chemistry Insect Proteins / genetics* Insect Proteins / metabolism Molecular Sequence Data Moths / genetics* Moths / metabolism Phylogeny Sequence Alignment |
| IF | 3.998 |
| Altmetric score |
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 9 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Silkworms | |